NOV 19, 2019 8:00 AM PST

Overcoming the Hurdles of Density Gradient Ultracentrifugation for Optimized Gene Therapy Purification Workflows

Speaker
  • Senior Applications Scientist - Beckman Coulter
    Biography
      Ross VerHeul holds a bachelor's degree in biology from Cleveland State University and conducted doctoral work in synthetic organic chemistry and chemical biology at Purdue University. His primary expertise has focused around the design and production of novel materials as inputs for precisely-controlled assembly of therapeutic nanoparticles for gene therapy. Along the way, Ross also developed significant wide-ranging expertise in microfluidic applications paired with the production, purification, and characterization of a multitude of biomaterials for in vitro and in vivo use. Such materials include synthetic and natural proteins, polymers, nanoparticles, and viral & non-viral vectors for gene therapy. Ross joined Beckman Coulter Life Sciences in January 2018 and is based in Indianapolis, Indiana where he leads application development for centrifugation.

    Abstract

    DATE: November 19, 2019

    TIME:  8:00am PST

    Centrifugation has long served as a critical separation tool in countless research and production facilities spanning a wide range of disciplines. Modern centrifuges reach incredibly high speeds (with centrifugal acceleration sometimes exceeding 1,000,000 x g) to aid the high-resolution separation of particles, typically in the micro- or nanometer range, by size and/or density. Today’s gene therapy products, such as viral vectors, require high-quality purification to ensure the consistent production of safe, efficacious therapeutics of the highest quality to further advance this rapidly growing field and deliver solutions to patients in need. 


    Density gradient ultracentrifugation (DGUC) is a centrifuge-based technique for providing superior purification of viral vectors (e.g., isolating full AAV particles from partial and empty capsids), along with other materials (such as plasmid DNA) in gene therapy production workflows. Though a well-established and mature method, DGUC is sometimes viewed as dated, challenging to design and conduct, or only suited for small-scale research applications. In this webinar, we’ll address these perceptions and discuss the premise of DGUC as a high-resolution purification technique for AAVs and plasmid DNA. We’ll also provide guidance on how to get started with DGUC and optimize this technique for gene therapy workflows. 

     

    Learning Objectives:

    • Learn about the various types of density gradients techniques, how they are used, and how to get started. 
    • Learn how to design and optimize a density gradient ultracentrifugation workflow, with AAV and plasmid DNA purification as examples. 
    • Learn how an optimized DGUC procedure can significantly increase throughput (by up to 7.5-fold) and become suitable for larger-scale workflows. 

     

    Webinars will be available for unlimited on-demand viewing after live event.

     

    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety. 


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