NOV 19, 2019 08:00 AM PST
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar

Overcoming the Hurdles of Density Gradient Ultracentrifugation for Optimized Gene Therapy Purification Workflows

Speakers
  • Senior Applications Scientist - Beckman Coulter
    Biography
      Ross VerHeul holds a bachelor's degree in biology from Cleveland State University and conducted doctoral work in synthetic organic chemistry and chemical biology at Purdue University. His primary expertise has focused around the design and production of novel materials as inputs for precisely-controlled assembly of therapeutic nanoparticles for gene therapy. Along the way, Ross also developed significant wide-ranging expertise in microfluidic applications paired with the production, purification, and characterization of a multitude of biomaterials for in vitro and in vivo use. Such materials include synthetic and natural proteins, polymers, nanoparticles, and viral & non-viral vectors for gene therapy. Ross joined Beckman Coulter Life Sciences in January 2018 and is based in Indianapolis, Indiana where he leads application development for centrifugation.

    Abstract:

    DATE: November 19, 2019

    TIME:  8:00am PST

    Centrifugation has long served as a critical separation tool in countless research and production facilities spanning a wide range of disciplines. Modern centrifuges reach incredibly high speeds (with centrifugal acceleration sometimes exceeding 1,000,000 x g) to aid the high-resolution separation of particles, typically in the micro- or nanometer range, by size and/or density. Today’s gene therapy products, such as viral vectors, require high-quality purification to ensure the consistent production of safe, efficacious therapeutics of the highest quality to further advance this rapidly growing field and deliver solutions to patients in need. 


    Density gradient ultracentrifugation (DGUC) is a centrifuge-based technique for providing superior purification of viral vectors (e.g., isolating full AAV particles from partial and empty capsids), along with other materials (such as plasmid DNA) in gene therapy production workflows. Though a well-established and mature method, DGUC is sometimes viewed as dated, challenging to design and conduct, or only suited for small-scale research applications. In this webinar, we’ll address these perceptions and discuss the premise of DGUC as a high-resolution purification technique for AAVs and plasmid DNA. We’ll also provide guidance on how to get started with DGUC and optimize this technique for gene therapy workflows. 

     

    Learning Objectives:

    • Learn about the various types of density gradients techniques, how they are used, and how to get started. 
    • Learn how to design and optimize a density gradient ultracentrifugation workflow, with AAV and plasmid DNA purification as examples. 
    • Learn how an optimized DGUC procedure can significantly increase throughput (by up to 7.5-fold) and become suitable for larger-scale workflows. 

     

    Webinars will be available for unlimited on-demand viewing after live event.

     

    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety. 


    Show Resources
    You May Also Like
    OCT 02, 2019 11:00 AM PDT
    OCT 02, 2019 11:00 AM PDT
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    JUN 19, 2019 10:00 AM PDT
    JUN 19, 2019 10:00 AM PDT
    DATE: June 19, 2019TIME: 10:00am PDT, 1:00pm EDT As we develop new methods to create more biologically relevant models for research in understanding disease etiology and in...
    OCT 01, 2019 08:00 AM PDT
    C.E. CREDITS
    OCT 01, 2019 08:00 AM PDT
    DATE: October 1, 2019TIME: 8:00am PTIn the webinar, Introduction to Characterization of Biomolecules Using Analytical Ultracentrifugation, we discussed the fundamentals of analytical...
    SEP 05, 2019 04:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 04:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    JUN 05, 2019 05:00 PM CEST
    C.E. CREDITS
    JUN 05, 2019 05:00 PM CEST
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    Loading Comments...
    Show Resources
    Event Countdown
    • 0 Days
    • 0 Hours
    • 0 Minutes
    • 0 Seconds