AUG 20, 2014 7:30 AM PDT

Panel Discussion: Increasing productivity and reproducibility by automating DNA library preparation for Ion Torrent Sequencing

Speaker

Abstract

Next Generation Sequencing has revolutionized genomic variant discovery. The major bottle-neck for sequencing projects is the individual, biological sample, library preparation. This platform specific addition of adaptor sequences to the target DNA of interest is often technically challenging and manually time-consuming. In the Genomics Shared Resource at the Geisel School of Medicine at Dartmouth, we have recently implemented automation of several of our DNA library preparation procedures in collaboration with Beckman Coulter. The NEBNext Fast Fragmentation Library Preparation for Ion Torrent sequencing was automated on the BioMek 4000 liquid handler. This automation allowed us to increase our turnaround times by 2 fold, and also increased the reproducibility of library preparations, and prevents the need for repeated preparations due to technical errors, which were not present in the automated method. Details of the automation methods, library characteristics and sequencing results will be presented. Additional automated methods have been developed for a custom cancer gene panel and whole exome sequencing libraries, the rationale and details for these will be discussed.


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AUG 20, 2014 7:30 AM PDT

Panel Discussion: Increasing productivity and reproducibility by automating DNA library preparation for Ion Torrent Sequencing



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