DEC 05, 2017 10:00 AM PST

WEBINAR: Pathologist's Perspective: Limitations and Benefits of Immunofixation and Immunotyping for Monoclonal Gammopathy Characterization

Sponsored by: Sebia
Speaker
  • Medical Director, CompuNet Clinical Labs and Miami Valley Hospital
    Biography
      Dr. Hood attended The Ohio State University and received his B.S. degree in microbiology/immunology in 1981. He graduated from Wright State University School of Medicine in 1985. Dr. Hood then completed an AP/CP residency program at the Cleveland Clinic Foundation (1985-1990) where he was chief resident for anatomic pathology July, 1987-June, 1988. During the last 18 months of his training he focused on clinical chemistry and hematology. Upon finishing his residency in 1990, Dr. Hood joined the staff of Miami Valley Hospital (a 750 bed tertiary care hospital) and CompuNet Clinical Labs (a regional reference lab) in Dayton, Ohio. Since 1997, Dr. Hood has been medical director of the clinical labs at Miami Valley Hospital and the CCL lab. During his career he has been on the boards of Miami Valley Hospital, Premier Health hospital system, Community Blood Center/Community Tissue Services, Ohio Society of Pathologists, and The Joint Restoration Foundation as well as serving as system pathology chair for Premier Health hospital system. Over 5 years ago, Dr. Hood transitioned the lab's protein electrophoresis from gel based to capillary zone electrophoresis.

    Abstract

    DATE: December 5, 2017
    TIME: 10:00AM PT, 1:00PM ET

     

    Monoclonal Gammopathies (MG) are characterized by chromosomal aberration of B-cells or plasma cells resulting in benign or malignant proliferation of the affected clone. The product of such clone, a monoclonal protein, is an essential laboratory marker for the detection, identification and classification of MG and aid in diagnosing the associated conditions. 

    Currently there is no one test sensitive and specific enough to detect all Monoclonal Gammopathies.  In some cases, multiple tests including serum free light chain analysis are required to uncover all plasma cell dyscrasias present in the patient.
      
    Two electrophoresis techniques commonly used in clinical laboratory for typing MGs are Immunofixation and Immunotyping.  During Immunofixation samples are separated on a gel using zone electrophoresis and separation products are overlaid with specific antisera for detection.  On capillary systems, MG can be characterized by mixing sample with Ig-specific antibodies followed by separation in an electrical field and observing resulting patterns for specific reduction of monoclonal peaks.

    Learning objectives:

    • Understand the principles of Immunotyping and Immunofixation
    • Review Immunotyping and Immunofixation results
    • Discuss triage strategies for detecting Monoclonal Gammopathies

     


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