JUL 12, 2018 6:00 PM PDT

Making Polychromatic Flow Cytometry easy after Instrument Characterization and Validation

Sponsored by: Thermo Fisher Scientific
Speaker
  • Flow Cytometry and Imaging Facility Manager, QIMR Berghofer Medical Research Institute
    Biography
      Grace has a strong instrumentation background and has been managing flow cytometry core facilities for over 30 years. She came to QIMR Berghofer in 1993 from her position as the Flow Cytometry Facility Manager at Peter MacCallum Cancer Institute in Melbourne. Previously she worked in clinical neurophysiology at the Alfred Hospital in Melbourne and also in the Neurosurgical Unit at The Maudsley Hospital and Institute of Psychiatry in London. She graduated from RMIT University with a Masters in Applied Science. Grace is active with the Australasian Cytometry Society (ACS) in organizing courses and meetings and currently holds position on the ACS executive committee. Grace has served two terms as Councilor on the Active International Society for Advancement of Cytometry (ISAC), she continues to be active with the society organizing workshops and being involved in teaching at ISAC cytometry courses. Her passion is teaching and she also runs flow cytometry workshops and courses in the Australasian region, she is also active in upskilling scientists in resource poor countries involved in using cytometry for HIV and malaria monitoring.

    Abstract

    DATE: July 12, 2018
    TIME: 6:00PM PDT

    Polychromatic flow cytometry is a powerful tool in measuring and quantitating the expression of numerous antigens present on a single cell. Different instrument configurations or instruments with similar configurations can result in varied results with some populations not being easily resolved. In this presentation, I will present experiments we have performed at QIMR Berghofer to help characterize our instruments and how they respond to different fluorochromes commonly used in flow cytometry. Using this knowledge enables us to better design multicolour panels for our facility users as well as our external clients. 

    Learning Objectives:

    • Learn to understand your flow cytometry instrument to determine individual instrument responses to different fluorochromes.
    • Calculate a spillover spreading matrix to aid in fluorochrome choice for multi-color design.
    • Better polychromatic panel design and fluorochrome choice for antigens of interest.


    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    APR 21, 2021 5:00 PM CEST
    APR 21, 2021 5:00 PM CEST
    Date: April 21, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Spatial Answers Trilogy - Spatial Answers in Immunology Immunology Researchers share their Spatial Discoveries in SARS-C...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    JUL 12, 2018 6:00 PM PDT

    Making Polychromatic Flow Cytometry easy after Instrument Characterization and Validation

    Sponsored by: Thermo Fisher Scientific

    Specialty

    Immunology

    Cell Biology

    Cancer Research

    Molecular Biology

    Cell Culture

    Biotechnology

    Antibodies

    Laboratory Testing

    Clinical Diagnostics

    Biochemistry

    Cancer Diagnostics

    Hematology

    Molecular Diagnostics

    Biomarkers

    Clinical Research

    Geography

    North America64%

    Europe17%

    Asia12%

    South America3%

    Oceania3%

    Registration Source

    Website Visitors81%

    WAVE Newsletter Promotion17%

    Trending Newsletter Promotion1%

    Email Promotions1%

    Job Title

    Medical Laboratory Technician15%

    Facility/Department Manager14%

    Research Scientist13%

    Scientist12%

    Student10%

    Executive7%

    Clinical Laboratory Scientist7%

    Educator/Faculty6%

    Post Doc5%

    Medical Doctor/Specialist4%

    Lab Management3%

    QC/QA2%

    Marketing/Sales1%

    Biologist1%

    Organization

    Academic Institution17%

    Clinical Laboratory15%

    Hospital15%

    Research Institute10%

    Biotech Company9%

    Government6%

    Life Science Company5%

    Medical School3%

    Pharmaceutical Company2%

    Medical Center2%

    Medical Device Company2%

    Non-Profit Organization2%

    Animal Hospital1%

    Media1%

    Other2%


    Show Resources
    Loading Comments...
    Show Resources
    Attendees
    • See more