JUL 12, 2018 9:00 AM PDT

Making Polychromatic Flow Cytometry easy after Instrument Characterization and Validation

Sponsored by: Thermo Fisher Scientific
Speaker

Abstract

DATE: July 12, 2018
TIME: 9:00AM PDT

Polychromatic flow cytometry is a powerful tool in measuring and quantitating the expression of numerous antigens present on a single cell. Different instrument configurations or instruments with similar configurations can result in varied results with some populations not being easily resolved. In this presentation, I will present experiments we have performed at QIMR Berghofer to help characterize our instruments and how they respond to different fluorochromes commonly used in flow cytometry. Using this knowledge enables us to better design multicolour panels for our facility users as well as our external clients. 

Learning Objectives:

  • Learn to understand your flow cytometry instrument to determine individual instrument responses to different fluorochromes.
  • Calculate a spillover spreading matrix to aid in fluorochrome choice for multi-color design.
  • Better polychromatic panel design and fluorochrome choice for antigens of interest.


Show Resources
You May Also Like
FEB 15, 2023 7:00 AM PST
FEB 15, 2023 7:00 AM PST
Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
NOV 16, 2022 7:00 PM PST
C.E. CREDITS
NOV 16, 2022 7:00 PM PST
Date: November 16, 2022 Time: 2:00pm (AEST) Date: November 17, 2022 7:00pm (PST), 10:00pm (EST), 4:00am (CET) The growth in FDA-approved cell and gene therapy products for the treatment of d...
NOV 01, 2022 10:00 AM PDT
NOV 01, 2022 10:00 AM PDT
Date: November 01, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 6:00pm (CET) Our laboratory developed the Pig-a gene mutation assay as a means to simply and reliably evaluate the potential mutage...
NOV 09, 2022 8:00 AM PST
C.E. CREDITS
NOV 09, 2022 8:00 AM PST
Date: November 09, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) The field of cell and gene therapy is rapidly growing. In particular, the use of lentiviruses in CAR-T applications is...
OCT 05, 2022 7:00 AM PDT
C.E. CREDITS
OCT 05, 2022 7:00 AM PDT
Date: October 05, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) Cancer is a complex disease at the level of both cells and tissues, and uncovering novel treatments requires understan...
JUL 12, 2018 9:00 AM PDT

Making Polychromatic Flow Cytometry easy after Instrument Characterization and Validation

Sponsored by: Thermo Fisher Scientific


Show Resources
Loading Comments...
Show Resources