JUL 12, 2018 9:00 AM PDT

Making Polychromatic Flow Cytometry easy after Instrument Characterization and Validation

Speaker
  • Flow Cytometry and Imaging Facility Manager, QIMR Berghofer Medical Research Institute
    Biography
      Grace has a strong instrumentation background and has been managing flow cytometry core facilities for over 30 years. She came to QIMR Berghofer in 1993 from her position as the Flow Cytometry Facility Manager at Peter MacCallum Cancer Institute in Melbourne. Previously she worked in clinical neurophysiology at the Alfred Hospital in Melbourne and also in the Neurosurgical Unit at The Maudsley Hospital and Institute of Psychiatry in London. She graduated from RMIT University with a Masters in Applied Science. Grace is active with the Australasian Cytometry Society (ACS) in organizing courses and meetings and currently holds position on the ACS executive committee. Grace has served two terms as Councilor on the Active International Society for Advancement of Cytometry (ISAC), she continues to be active with the society organizing workshops and being involved in teaching at ISAC cytometry courses. Her passion is teaching and she also runs flow cytometry workshops and courses in the Australasian region, she is also active in upskilling scientists in resource poor countries involved in using cytometry for HIV and malaria monitoring.

    Abstract

    DATE: July 12, 2018
    TIME: 9:00AM PDT

    Polychromatic flow cytometry is a powerful tool in measuring and quantitating the expression of numerous antigens present on a single cell. Different instrument configurations or instruments with similar configurations can result in varied results with some populations not being easily resolved. In this presentation, I will present experiments we have performed at QIMR Berghofer to help characterize our instruments and how they respond to different fluorochromes commonly used in flow cytometry. Using this knowledge enables us to better design multicolour panels for our facility users as well as our external clients. 

    Learning Objectives:

    • Learn to understand your flow cytometry instrument to determine individual instrument responses to different fluorochromes.
    • Calculate a spillover spreading matrix to aid in fluorochrome choice for multi-color design.
    • Better polychromatic panel design and fluorochrome choice for antigens of interest.


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