POSTER: Alternative Blotting Methods

Speaker
  • Research Scientist, Protein and Cell Analysis, Thermo Fisher Scientific
    Biography
      Bea is a R&D scientist in the protein analysis group. She completed her BS at Rockford University in 1997 in chemistry. She obtained her MS in medical biotechnology from the University of Illinois College of Medicine at Rockford in 2010. Over the past 20 years, she has worked on the development of numerous products for the life science research market. She has extensive experience in electrophoresis, protein transfer and detection, western blot and gel imaging, protein sample preparation, fluorescent labeling and purification of proteins and antibodies, dialysis, and protein staining and transfer devices.

    Abstract

    Western blotting is an important technique for characterizing specific protein targets. Successful western detection is dependent on the quality of protein transfer from polyacrylamide gels to the blotting membranes, and on the processing steps and reagents used. The traditional wet transfer method offers high transfer efficiency, but at a cost of time and effort. Improvements to dry and semi-dry transfer systems have greatly improved the transfer speed (less than 10 min) and convenience without compromising transfer efficiency. This poster will discuss the traditional wet blotting transfer technique as compared to dry and semi-dry transfer techniques.

    Learning Objectives:

    • Compare the classical wet western blotting technique to dry and semi-dry blotting techniques
    • Learn about dry and semi-dry western blotting for high-efficiency transfer and quality results

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