POSTER: Alternative Blotting Methods

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Research Scientist, Protein and Cell Analysis, Thermo Fisher Scientific
    Biography
      Bea is a R&D scientist in the protein analysis group. She completed her BS at Rockford University in 1997 in chemistry. She obtained her MS in medical biotechnology from the University of Illinois College of Medicine at Rockford in 2010. Over the past 20 years, she has worked on the development of numerous products for the life science research market. She has extensive experience in electrophoresis, protein transfer and detection, western blot and gel imaging, protein sample preparation, fluorescent labeling and purification of proteins and antibodies, dialysis, and protein staining and transfer devices.

    Abstract

    Western blotting is an important technique for characterizing specific protein targets. Successful western detection is dependent on the quality of protein transfer from polyacrylamide gels to the blotting membranes, and on the processing steps and reagents used. The traditional wet transfer method offers high transfer efficiency, but at a cost of time and effort. Improvements to dry and semi-dry transfer systems have greatly improved the transfer speed (less than 10 min) and convenience without compromising transfer efficiency. This poster will discuss the traditional wet blotting transfer technique as compared to dry and semi-dry transfer techniques.

    Learning Objectives:

    • Compare the classical wet western blotting technique to dry and semi-dry blotting techniques
    • Learn about dry and semi-dry western blotting for high-efficiency transfer and quality results

    Show Resources
    You May Also Like
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    MAY 08, 2020 10:00 AM PDT
    C.E. CREDITS
    MAY 08, 2020 10:00 AM PDT
    DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
    MAY 12, 2020 9:00 AM PDT
    C.E. CREDITS
    MAY 12, 2020 9:00 AM PDT
    NEW DATE: Date: May 12, 2020 Time: 9:00am PDT, 12:00pm EDT...
    APR 20, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 20, 2020 8:00 AM PDT
    This talk will cover different approaches to emergency hemostasis testing for patients that are actively bleeding due to trauma, surgery, obstetrical emergencies and other causes. The talk i...
    DEC 05, 2019 5:00 AM PST
    C.E. CREDITS
    DEC 05, 2019 5:00 AM PST
    DATE: December 5, 2019TIME: 5:00am PST, 8:00am EST The diagnosis of an active infection by the hepatitis B virus (HBV) relies on testing for the HBV surface antigen (HBsAg). Ov...
    Loading Comments...
    Show Resources