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Practical Considerations and Best Practices for Single Cell and Spatial Transcriptomics

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Staff Field Application Scientist, 10X Genomics
    Biography

      Neil Winegarden is a Field Application Scientist with 10x Genomics. Before joining 10x in 2018, Neil was the Head of Operations for a leading genomics core in Toronto, Canada, where he worked for 20 years. Neil's interest in single cell genomics dates back to 2002 having collaborated with Dr. Norman Iscove to demonstrate profiling single cells on gene expression microarrays. Neil was a 10x customer for two years before he decided to join the company. He now enthusiastically supports customers in both Canada and the US.


    Abstract

    Traditional genomics approaches look at bulk signatures from often heterogeneous samples or tissues. These approaches do not have the requisite resolution to fully uncover the true complexity of biology. Single cell and spatial transcriptomics approaches provide the ability to build a more comprehensive multidimensional understanding of complex biological systems. In order to fully utilize such technologies, there are several key experimental design and sample preparation considerations that when followed will help lead to success.  Both single cell and spatial transcriptomics provide high resolution views, and we will discuss how combining both of these technologies work synergistically to uncover an even greater understanding.

    Learning Obejctives:

    1. Understand the key requirements for sample preparation in a single cell and/or spatial transcriptomics experiment

    2. Learn the synergies between a combined single cell and spatial transcriptomics approach


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