OCT 24, 2019 7:30 AM PDT

Quantitation of Mycotoxins in Food Matrices Comparing Stable Isotope Dilution Assay (SIDA) with Matrix Matched Calibration Methods by LC-MS/MS

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Application Scientist at Restek
    Biography
      Dan Li is an Application Scientist at Restek. She received her PhD degree in analytical chemistry under the supervision of Professor Milton Lee at Brigham Young University. Her work focuses on pharmaceutical, food, and environmental applications using LC-MS and GC-MS. She was working at Covance performing bioanalytical method development and validation before she joined Restek in 2015.

    Abstract

    Mycotoxins are secondary fungal metabolites produced by mold that may be found in food or feed. They can cause severe health problems in humans and animals, and can result in significant economic losses. Among the hundreds of toxic mycotoxins, aflatoxins, fumonisins, deoxynivalenol, ochratoxin A, HT-2 toxin, zearalenone, and T-2 toxin are considered as a major concern for corn, wheat, peanuts and other agricultural products. LC-MS has become the standard and is now widely used for routine mycotoxin analysis and identification.  One of the challenges faced by LC-MS techniques is the matrix effects caused by the use of electro-spray ionization (ESI).  Generally, sample preparation, chromatographic and calibration techniques are the common strategies for reducing the negative effects of matrix effects.  Standard addition, matrix matching, and stable isotope dilution assay (SIDA) are all possible calibration solutions.  In this work, a quick “dilute-filter-shoot” method was used for sample preparation.  A seven-minute LC-MS/MS method using a biphenyl phase column was developed and verified for quantifying twelve mycotoxins in four commodities: corn, peanut butter, brown rice, and corn & wheat mixed.  Both SIDA and matrix matched calibration methods were applied, compared, and evaluated in terms of recovery, efficiency, advantages, and limitations.


    Show Resources
    You May Also Like
    JUN 09, 2020 10:00 AM PDT
    C.E. CREDITS
    JUN 09, 2020 10:00 AM PDT
    DATE: June 9, 2020 TIME: 10:00am PT, 1:00pm ET The presentation will first discuss sepsis as a disease and then explain the importance of performing diagnostic tests in the clinical labora...
    APR 27, 2020 9:00 AM PDT
    C.E. CREDITS
    APR 27, 2020 9:00 AM PDT
    DATE: April 27, 2020 TIME: 9:00am PT, 12:00pm ET Recombinant adeno-associated virus (rAAV) is a small (25-nm) virus that is composed of a protein shell called a capsid and a single-stranded...
    MAY 04, 2020 9:00 AM PDT
    C.E. CREDITS
    MAY 04, 2020 9:00 AM PDT
    DATE: May 4, 2020 TIME: 9:00am PT, 12:00pm ET...
    JUN 02, 2020 9:00 AM PDT
    C.E. CREDITS
    JUN 02, 2020 9:00 AM PDT
    DATE: June 2, 2020 TIME: 9:00am PT, 12:00pm ET Osmolality is a well-established parameter for upstream bioprocessing, formulation, analytical development and QC applications throughout the m...
    MAR 27, 2020 11:00 AM PDT
    C.E. CREDITS
    MAR 27, 2020 11:00 AM PDT
    DATE: March 27, 2020 TIME: 11:00am PT, 2:00pm ET Adeno-associated virus (AAV) is one of the most commonly used delivery vehicles in gene therapy development. However, the presence of product...
    FEB 14, 2020 10:00 AM PST
    C.E. CREDITS
    FEB 14, 2020 10:00 AM PST
    DATE: February 14, 2020 TIME: 10:00am PT, 1:00pm ET Separation challenges arise in the lab on a daily basis and you rely on a broad analytical toolset to help solve them. In this presentatio...
    Loading Comments...
    Show Resources
    Attendees
    • See more