APR 29, 2020 8:00 AM PDT

Realize better NGS results through gentle cell sorting

Sponsored by: Miltenyi Biotec
Speakers
  • Graduate Student Researcher, PhD Candidate Department of Biological Chemistry University of California, Irvine - School of Medicine
    BIOGRAPHY
  • Global Product Manager, Cell Sorting, Miltenyi Biotec B.V. & Co KG
    BIOGRAPHY

Abstract
DATE:  April 29, 2020
TIME:   8:00am PT, 11:00am ET

Single cell genomics and other next generation sequencing applications depend strongly on adequate upstream sample preparation. Results can easily be affected by poor cellular viabilities, the presence of non-target cells or debris, as well as by a high contaminating background of free DNA or RNA. In addition, harsh conditions during sample preparation workflows can easily cause alterations to transcriptome, and thereby generate unwanted artifacts. This is especially troublesome if you are interested in genes related to cellular stress, activation, or metabolism. A solution to this is to make use of only non-stressed, viable target cells of high purity in your genomics experiments. The trick is getting them.

In this webinar you will learn:

  • Which sample preparation steps can introduce artifacts in your NGS data and how to best avoid common pitfalls, in particular when cell sorting is required prior to analysis
  • How this critical cell sorting step can be designed to be more gentle and have less of an impact on cells by using the right technology
  • How other scientists have mastered cell sorting and gained high quality data

 

Webinars will be available for unlimited on-demand viewing after the live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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