APR 29, 2020 8:00 AM PDT

Realize better NGS results through gentle cell sorting

Sponsored by: Miltenyi Biotec
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Graduate Student Researcher, PhD Candidate Department of Biological Chemistry University of California, Irvine - School of Medicine
    Biography
      Quy received his B.S. in Biochemistry and Molecular Biology from the University of California, Irvine. He is currently a PhD candidate at the University of California, Irvine in Dr. Kai Kessenbrock's Lab. He is focused on identifying and characterizing the spectrum of heterogeneity within the breast epithelium on the single cell level to elucidate the changes that occur during breast cancer initiation and progression.
    • Global Product Manager, Cell Sorting, Miltenyi Biotec B.V. & Co KG
      Biography
        As a global product manager, Felix is responsible for the development of applications, new protocols, and products for the MACSQuant® Tyto® Cell Sorter from Miltenyi Biotec. Before joining Miltenyi Biotec in 2018, Felix worked as a research associate at the LIMES Institute, University of Bonn, Germany, with a strong focus on Molecular Immunology and Cell Biology. He obtained his PhD in Molecular Biomedicine from the University in Bonn in 2017.

      Abstract
      DATE:  April 29, 2020
      TIME:   8:00am PT, 11:00am ET

      Single cell genomics and other next generation sequencing applications depend strongly on adequate upstream sample preparation. Results can easily be affected by poor cellular viabilities, the presence of non-target cells or debris, as well as by a high contaminating background of free DNA or RNA. In addition, harsh conditions during sample preparation workflows can easily cause alterations to transcriptome, and thereby generate unwanted artifacts. This is especially troublesome if you are interested in genes related to cellular stress, activation, or metabolism. A solution to this is to make use of only non-stressed, viable target cells of high purity in your genomics experiments. The trick is getting them.

      In this webinar you will learn:

      • Which sample preparation steps can introduce artifacts in your NGS data and how to best avoid common pitfalls, in particular when cell sorting is required prior to analysis
      • How this critical cell sorting step can be designed to be more gentle and have less of an impact on cells by using the right technology
      • How other scientists have mastered cell sorting and gained high quality data

       

      Webinars will be available for unlimited on-demand viewing after the live event.
       
      LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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