NOV 12, 2020 10:30 AM PST

Recovery of cfDNA using the QIAsymphony DSP Circulating DNA Kit

Sponsored by: QIAGEN
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Senior Scientist
    Biography

      Alexander Wolf is a Senior Scientist in Sample Preparation Technologies, Molecular Diagnostics Development at QIAGEN GmbH. Following completion of a PhD in Biochemistry and a 3-year post-doctoral fellowship at the Institute of Pharmacology Giessen, Germany, Dr. Wolf joined QIAGEN in 2012 and has focused on the liquid biopsy portfolio for the past 8 years. As technical lead of the Molecular Diagnostics Development team, he was mainly responsible for the development of the QS DSP Circulating DNA Kit which launched in 2017 and remains dedicated to customer support of this product today.


    Abstract

    Cell-free DNA (cfDNA) is a key analyte for liquid biopsy samples. Due to extremely low concentration and high degree of fragmentation, the extraction of cfDNA is technically challenging. Here, the efficiency of the automated large volume cfDNA extraction method was evaluated with regard to different performance parameters like quantitative cfDNA extraction for total and low-abundant biomarkers with focus on small fragments, precision, linearity for a broad range of input volumes and compatibility to downstream assays.

    Learning Objectives:

    1. Handling & challenges with cfDNA

    2. Embedment of cfDNA extraction workflow into customized downstream assay

    3. Quality control of extracted cfDNA


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