MAR 16, 2017 10:30 AM PDT

Refinement of epigenetic approaches in neuroscience

Presented at: Neuroscience 2017
Sponsored by: Agilent
Speaker
  • Assistant Professor, Johns Hopkins University School of Medicine
    Biography
      Richard Lee is a Johns Hopkins University "lifer." He received his B.S. in Biomedical Engineering and a Ph.D. in Molecular Biology and Genetics at the Johns Hopkins University. He was trained in epigenetics under the mentorship of Dr. Andrew Feinberg and completed his postdoctoral fellowship in the Department of Psychiatry at the Johns Hopkins School of Medicine. Currently, he is an assistant professor in the Department of Psychiatry, and his main research interest is in elucidating the epigenetic mechanisms of the HPA axis and neuroendocrine hormones in the context of psychiatric disorders. Dr. Lee demonstrated that stress hormones or glucocorticoids can directly alter DNA methylation in vivo. He employs both candidate loci and genome-wide approaches to identify neuronal genes and pathways that are epigenetically affected by stress and glucocorticoids. He has designed and implemented both microarray and sequencing based platforms for genome-wide DNA methylation projects, including the CHARM (Comprehensive High-throughput Arrays for Relative Methylation) platform and the SureSelect Methyl-Seq Target Enrichment System for the rat genome. He is currently refining epigenetic approaches to better study specific neuronal populations by implementing powerful tools in genetics and neuroscience.

    Abstract

    Epigenetics refers to the study of nuclear architecture and gene regulation. Epigenetic mechanisms govern many physiological processes such as cell differentiation, x-inactivation, and genomic imprinting, and pathophysiological processes such as loss of imprinting. Epigenetic mechanisms are especially relevant in psychiatry and neuroscience, as exposure to stress, trauma, or toxicants are thought to alter brain function through epigenetic mechanisms. However, there are substantial challenges to studying epigenetics in the brain, as the brain is a highly heterogeneous organ whose constituent cells and circuits perform diverse functions such as storage of memory, behavioral and emotional response, and regulation of homeostasis among many others.

    In this presentation, Dr. Lee will first provide a brief overview of epigenetics and several commonly used approaches to study epigenetic mechanisms in gene regulation. In particular, he will focus on the implementation of an unbiased, genome-wide platform called Methyl-Seq to study DNA methylation, which is one of the more commonly studied epigenetic modifications, to identify targets of stress and glucocorticoid exposure in the brain. Dr. Lee will then discuss some of the challenges associated with implementing epigenetic tools to answer questions in psychiatry and neuroscience, such as limiting starting material, cellular heterogeneity, and neuronal subtypes. Dr. Lee will also focus on enrichment methods using genetic labeling and flow cytometry to isolate relatively homogenous populations of cells for downstream epigenetic applications. Epigenetic tools, when combined with concepts and powerful analytical tools in neuroscience, have the potential to elucidate the molecular underpinnings of neuronal function.

     

    For Research Use Only.  Not for use in diagnostic procedures.


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