Date: June 09, 2022
Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST)
It is known that the immune response in pregnant women undergoes recognized changes that ensure the mother does not reject the fetus. Dysregulation of this dynamic immune adaptation underpins numerous adverse pregnancy outcomes such as miscarriage, preeclampsia, and preterm birth. These normal changes in the immune response also make pregnant women more susceptible to severe infections of influenza, measles, and hepatitis E and affect clinical symptoms of autoimmune diseases. Despite the knowledge of these immune response changes, little is known about how immune cells undergo functional changes during pregnancy.
Here, we show how a high purity automated isolation from blood and tissue mononuclear phagocytes allow for an accurate investigation to further understand how these changes occur at the cellular level. Flow cytometry, bioenergetics analysis and transcriptomics were used to monitor the immunometabolic profile of mononuclear phagocytes during pregnancy. We can apply this knowledge to other immune cell populations and also to bridge the many gaps in the basic, translational, and clinical immunology of pregnancy.
- Discuss the characterization of monocyte phenotype and function from subsets to mitochondria
- Describe how tissue macrophages respond to their environment
- Explain how defined cytokine exposures generate discrete macrophage types
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