Robust and Reproducible Protein Quantification in Plasma using Evosep One and Agilent 6495 LC/TQ

Here we showcase the reproducibility, robustness, and analytical sensitivity of MRM-based LC/MS analysis of human plasma proteins using the Evosep One LC system and Agilent 6495 triple quadrupole LC/MS with an Agilent nanospray source. Results demonstrated excellent retention time reproducibility over a 12-day analysis period, and equivalent sensitivity before and after robustness testing, underlining the suitability of the system for high-throughput protein quantification when sample amounts were limited.

For Research Use Only. Not for use in diagnostic procedures.