Date: February 22, 2023
Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET)
Circular plasmid DNA has traditionally been used as a stable and efficient vector for gene expression and HDR-mediated gene insertion. These vectors typically carry at least one antibiotic-resistant gene to ensure that the positive clone is accurately selected and genetically stable. However, for therapeutic applications, introducing antibiotic-resistant genes via exogenous plasmid DNA risks integration into the human genome causing resistance in patients. Therefore, eliminating the presence of antibiotic-resistance genes in the manufacture of plasmid DNA has been of interest.
Additionally, smaller-sized plasmid DNA significantly improves transfection and expression efficiency. However, a reduced backbone poses challenges when manufacturing circular dsDNA.
In this presentation, we will introduce GenCircle, our newly developed proprietary miniature circular dsDNA. GenCircle dsDNA is antibiotic resistance gene free with only 429bp backbone size and an easily scalable manufacturing process. We will also discuss how to best design your GenCircle dsDNA construct, as well as data on its improved performance in T cell engineering and in vivo expression.
- Introduce GenCircle dsDNA and describe the unique benefits (small vector size, antibiotic resistance gene free, flexible insert gene size)
- Demonstrate 3 main uses and show how to design your constructs with GenCircle vector. (Gene insert template, viral packaging plasmid, GOI expression vector)
- Show data comparing GenCircle dsDNA to traditional plasmid vectors
- Compare GenCircle dsDNA to other GenScript HDR template formats (GenExact linear ssDNA, GenWand linear dsDNA )
Webinars will be available for unlimited on-demand viewing after live event.
Labroots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.