SEP 12, 2019 10:00 AM PDT

High Sensitivity Purity Analysis of Adeno-Associated Virus (AAV) Capsid Proteins using CE-SDS

Sponsored by: SCIEX
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract
DATE:   September 12, 2019
TIME:    10:00am PDT, 1:00pm EDT
 
 
Adeno-associated virus (AAV) is one of the most widely used gene delivery vehicles for gene therapy because of its non-pathogenicity, low immunogenicity and different tropism to multiple cell types. Purity analysis of AAV viral proteins is important for quality assurance and the safety of AAV products.
 
In this webinar, Tingting Li, Sr. BioPharma Application Development Scientist, will demonstrate the capability of the CE-SDS method for purity analysis of AAV viral proteins with straight forward and easy sample preparation, excellent resolving power, good repeatability and linearity of absorbance response to sample concentration.
 
The gold standard CE-SDS chemistry from SCIEX, traditionally used for mAbs, can provide automated separation of AAV capsid viral proteins with higher resolution, quantitation capability, better reproducibility and is less labor intensive than traditional SDS-PAGE.
 
The CE-SDS method using UV or PDA detector and stacking injection technology provides good results for AAV samples with higher titers. For in-process AAV product analysis, which requires higher sensitivity, a Laser Induced Fluorescence detection method may be used with great reproducibility.
 
 
Learning Objectives:
  • Straightforward and easy sample preparation procedure using gold standard CE-SDS chemistry 
  • Flexibility with 3 possible detection modes, including high sensitivity CE-LIF for low titer samples
  • Achieve excellent resolution of VP1, VP2 and VP3 capsid proteins with better quantitation capability than SDS-PAGE
 
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

 

 

 


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
DEC 16, 2020 8:00 AM PST
C.E. CREDITS
DEC 16, 2020 8:00 AM PST
Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
MAR 16, 2021 10:00 AM PDT
C.E. CREDITS
MAR 16, 2021 10:00 AM PDT
Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
JUL 15, 2021 9:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
JUL 15, 2021 9:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
MAR 25, 2021 8:00 AM PDT
C.E. CREDITS
MAR 25, 2021 8:00 AM PDT
Date: March 25, 2021 Time: 8:00am (PST), 11:00am (EST) Flow cytometry has expanded to have more users, more data, and multiple instrument types. While this has lead to more quantitative data...
SEP 12, 2019 10:00 AM PDT

High Sensitivity Purity Analysis of Adeno-Associated Virus (AAV) Capsid Proteins using CE-SDS

Sponsored by: SCIEX
C.E. Credits: P.A.C.E. CE Florida CE

Specialty

Molecular Biology

Biotechnology

Cell Biology

Biochemistry

Immunology

Cell Culture

Laboratory Testing

Dna

Cancer Research

Microbiology

Genetics

Molecular Diagnostics

Pcr/rt-Pcr/real-Time Pcr

Infectious Disease

Gene Expression

Geography

North America58%

Asia17%

Europe14%

South America5%

Africa2%

Registration Source

Website Visitors56%

Email Promotions27%

Client/Sponsor Promotions6%

WAVE Newsletter Promotion5%

Trending Newsletter Promotion3%

Other2%

Job Title

Scientist21%

Student13%

Research Scientist10%

Executive10%

Medical Laboratory Technician10%

Facility/Department Manager7%

Lab Management5%

Educator/Faculty5%

Chemist3%

Medical Doctor/Specialist3%

QC/QA3%

Marketing/Sales3%

Clinical Laboratory Scientist3%

Biologist2%

Post Doc1%

Consultant1%

Organization

Academic Institution19%

Biotech Company14%

Hospital9%

Pharmaceutical Company8%

Research Institute6%

Clinical Laboratory5%

Contract Research Organization (Cro)5%

Government4%

Life Science Company4%

Distribution/sales3%

Consultant2%

Medical Center2%

Non-Profit Organization2%

Manufacturer & Biotech/pharma2%

Other2%


Show Resources
Loading Comments...
Show Resources
Attendees
  • See more