SEP 12, 2019 10:00 AM PDT

High Sensitivity Purity Analysis of Adeno-Associated Virus (AAV) Capsid Proteins using CE-SDS

SPONSORED BY: SCIEX
Speakers
  • Sr. BioPharma Application Development Scientist, SCIEX
    Biography
      Tingting Li obtained her MS degree at Rutgers University in Chemistry. Tingting worked in R&D and QC labs as Scientist in pharmaceutical companies for about 4.5 years on analytical method development and validation in NJ, US and worked in R&D lab as lab manager in NGS (Next generation sequencing) industry in China for about 1 year. Tingting Li joined Beckman Coulter (Shanghai, China) as Senior Product Specialist supporting the CE product line since 01/01/2013, then transferred to Sciex (Shagnhai, China) as a member of CE Separation team since 01/01/2014. Tingting moved to United States and joined application team in Sciex as a contractor in Sciex-Brea from 08/2017 for about half a year and then was hired as Senior Biopharma Application Development Scientist in Biologics team in Sciex (Brea) since 03/2018.

    Abstract:
    DATE:   September 12, 2019
    TIME:    10:00am PDT, 1:00pm EDT
     
     
    Adeno-associated virus (AAV) is one of the most widely used gene delivery vehicles for gene therapy because of its non-pathogenicity, low immunogenicity and different tropism to multiple cell types. Purity analysis of AAV viral proteins is important for quality assurance and the safety of AAV products.
     
    In this webinar, Tingting Li, Sr. BioPharma Application Development Scientist, will demonstrate the capability of the CE-SDS method for purity analysis of AAV viral proteins with straight forward and easy sample preparation, excellent resolving power, good repeatability and linearity of absorbance response to sample concentration.
     
    The gold standard CE-SDS chemistry from SCIEX, traditionally used for mAbs, can provide automated separation of AAV capsid viral proteins with higher resolution, quantitation capability, better reproducibility and is less labor intensive than traditional SDS-PAGE.
     
    The CE-SDS method using UV or PDA detector and stacking injection technology provides good results for AAV samples with higher titers. For in-process AAV product analysis, which requires higher sensitivity, a Laser Induced Fluorescence detection method may be used with great reproducibility.
     
     
    Learning Objectives:
    • Straightforward and easy sample preparation procedure using gold standard CE-SDS chemistry 
    • Flexibility with 3 possible detection modes, including high sensitivity CE-LIF for low titer samples
    • Achieve excellent resolution of VP1, VP2 and VP3 capsid proteins with better quantitation capability than SDS-PAGE
     
     
     
    Webinars will be available for unlimited on-demand viewing after live event.
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

     

     

     


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