AUG 21, 2014 9:45 AM PDT

Shotgun sequencing enables non-invasive monitoring of infection and rejection in transplantation

Speaker
  • Postdoctoral researcher, HHMI research associate, Departments of Bioengineering and Applied Physics, Stanford University
    Biography

      Iwijn De Vlaminck obtained a PhD from the KuLeuven in Belgium. He has a background in applied physics, specifically in nanoscale optics and mechanics. After his PhD he joined the lab of Cees Dekker at the TUDelft as a postdoc in single-molecule biophysics. He joined the lab of Stephen Quake at Stanford University as a postdoctoral researcher in 2012. His current interests include single-cell genomics and sequencing-based medical diagnostics.
       

    Abstract

    Accurate and timely diagnosis of rejection and infection is essential for long-term survival of solid-organ transplant recipients. We evaluated the performance a novel test to monitor rejection and infection through shotgun sequencing of plasma-derived cell-free DNA. We show that an elevated level of donor-derived cell-free DNA correlates with indicators of graft dysfunction and rejection in heart and lung transplantation. We also find a strong correlation between clinical test results and cell-free DNA derived from cytomegalovirus (CMV), the primary infectious disease complication for transplant recipients. We further show that cell-DNA can be used to detect and monitor pathogens that are not routinely screened in the clinic and we show that the composition of viral sequences is highly dynamic and strongly affected by immunosuppressive and antiviral drugs.


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    AUG 21, 2014 9:45 AM PDT

    Shotgun sequencing enables non-invasive monitoring of infection and rejection in transplantation


    Specialty

    Clinical Microbiology

    Dna Sequencing

    Personalized Medicine

    Clinical Diagnostics

    Immunity

    Cancer Diagnostics

    Immunotherapy

    Bioinformatics

    Flow Cytometry

    Antibiotics

    Immuno-Oncology

    Human Genetics

    Gene Expression

    Dna

    Big Data

    Geography

    North America50%

    Europe50%

    Registration Source

    Website Visitors100%

    Job Title

    Student50%

    Medical Laboratory Technician50%

    Organization

    Medical Center50%

    Academic Institution50%


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