AUG 21, 2014 09:45 AM PDT

Shotgun sequencing enables non-invasive monitoring of infection and rejection in transplantation

Speakers
  • Postdoctoral researcher, HHMI research associate, Departments of Bioengineering and Applied Physics, Stanford University
    Biography

      Iwijn De Vlaminck obtained a PhD from the KuLeuven in Belgium. He has a background in applied physics, specifically in nanoscale optics and mechanics. After his PhD he joined the lab of Cees Dekker at the TUDelft as a postdoc in single-molecule biophysics. He joined the lab of Stephen Quake at Stanford University as a postdoctoral researcher in 2012. His current interests include single-cell genomics and sequencing-based medical diagnostics.
       

    Abstract:

    Accurate and timely diagnosis of rejection and infection is essential for long-term survival of solid-organ transplant recipients. We evaluated the performance a novel test to monitor rejection and infection through shotgun sequencing of plasma-derived cell-free DNA. We show that an elevated level of donor-derived cell-free DNA correlates with indicators of graft dysfunction and rejection in heart and lung transplantation. We also find a strong correlation between clinical test results and cell-free DNA derived from cytomegalovirus (CMV), the primary infectious disease complication for transplant recipients. We further show that cell-DNA can be used to detect and monitor pathogens that are not routinely screened in the clinic and we show that the composition of viral sequences is highly dynamic and strongly affected by immunosuppressive and antiviral drugs.


    Show Resources
    You May Also Like
    SEP 05, 2019 04:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 04:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    MAY 16, 2019 04:00 PM CEST
    C.E. CREDITS
    MAY 16, 2019 04:00 PM CEST
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    OCT 02, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    OCT 02, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    AUG 13, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 13, 2019 09:00 AM PDT
    DATE: August 13, 2019TIME: 9:00am PT, 12:00pm ET, 5:00pm BST Molecular complexes are major constituents of cells, hence unraveling their mechanisms is key to fuller comprehension of c...
    JUN 19, 2019 10:00 AM PDT
    JUN 19, 2019 10:00 AM PDT
    DATE: June 19, 2019TIME: 10:00am PDT, 1:00pm EDT As we develop new methods to create more biologically relevant models for research in understanding disease etiology and in...
    Loading Comments...
    Show Resources