SEP 17, 2015 08:00 AM PDT

WEBINAR: Significantly simplify your plant metabolomics workflow

SPONSORED BY: Sciex, Sciex
C.E. CREDITS: CE
Speakers
  • Business Development Manager, SCIEX, EMEA
    Biography
      Dr. Stephen Lock obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry. Previously a lecturer of Chemistry at the University of Hull and a senior scientist at CCFRA he has worked for SCIEX™ in various technical roles for over 16 years. He has over 25 years' experience in Analytical Chemistry and has presented at over 50 international meeting around the world. Dr. Lock is currently the EMEA Business Development Manager for the Separation Division of SCIEX.

    Abstract:

    Despite recent developments in hyphenated techniques and column technology, analysis of small polar analytes (small organic acids, phosphorylated sugars, and underivatized amino acids) remains challenging.  These small charged metabolites are rarely retained on standard reverse phase columns and usually require HILIC/Hypercarb columns or ion chromatography.  In ion chromatography run times are usually long, Hypercarb columns usually need extensive conditioning before samples are run and to maintain peak shape HILIC chromatography often requires that extracts contains organic solvents such as acetonitrile which may not be the best solvent for highly polar compounds.  All of these techniques require different mobile phases or columns when you move between positive and negatively charged metabolites.

    In this webinar presentation, we present an alternative workflow, called CESI-MS, which not only overcomes these stationary-phase-based separation challenges, but is also capable of detecting these polar metabolites at physiological levels without the need for derivatization.  We will show how this technique can also be used to separate isobaric and structurally similar metabolites including phosphorylated sugars.



    Key Learning Points:



    • Fast, robust, and reliable method for high-quality metabolomic fingerprinting of both cationic and anionic metabolites without the need for derivatization.

    • How CESI-MS allows instant switching between negative and positive ionization modes without the need to change capillary or buffers.

    • Methods to separate isobaric and structurally similar metabolites based on their charge & hydrodynamic shape.


    Show Resources
    You May Also Like
    AUG 30, 2018 11:00 AM EDT
    C.E. CREDITS
    AUG 30, 2018 11:00 AM EDT
    DATE: August 30, 2018TIME: 11:00am EDT, 5:00pm CEST The need to quantify urine is valuable because it provides information on chemicals that the body makes naturally, as well...
    AUG 30, 2018 10:00 AM CEST
    C.E. CREDITS
    AUG 30, 2018 10:00 AM CEST
    DATE: August 30, 2018TIME:  10:00am CEST, 4:00pm CST The need to quantify urine is valuable because it provides information on chemicals that the body makes naturall...
    DEC 11, 2018 10:00 AM PST
    C.E. CREDITS
    DEC 11, 2018 10:00 AM PST
    DATE: December 11, 2018TIME: 11:00 PSTThis webinar will focus on how Reichert's Surface Plasmon Resonance (SPR) systems can generate additional valuable information that is not...
    JUN 28, 2017 07:00 AM PDT
    JUN 28, 2017 07:00 AM PDT
    DATE: June 28, 2017TIME: 7:00am PT, 10am ETAs gene therapy approaches usually require large amounts of AAV vectors for clinical use, few manufacturing processes have been repo...
    SEP 12, 2017 08:00 AM PDT
    SEP 12, 2017 08:00 AM PDT
    DATE: September 12, 2017TIME: 8:00am PT, 11:00am ETEvery year, millions of dollars are wasted on poorly characterized and performing antibodies.  Key researchers in the antibody co...
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    Loading Comments...