SEP 17, 2015 8:00 AM PDT

WEBINAR: Significantly simplify your plant metabolomics workflow

Sponsored by: Sciex, Sciex
Speaker
  • Biopharma EMEAI Marketing and Market Development Manager, Sciex. Scientific Advisor, Horváth Csaba Memorial Laboratory, University of Pannonia.
    Biography
      Dr. Stephen Lock obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry. Previously a lecturer of Chemistry at the University of Hull and a senior scientist at CCFRA he has worked for SCIEX™ in various technical roles for over 20 years. He has over 25 years’ experience in Analytical Chemistry and has presented at over 80 international meetings around the world.
      Steve is currently the EMEA BioPharma Marketing and Market Development Manager of SCIEX and is also an Adjunct Professor at the University of Pannonia where he acts in a volunteer role as the Scientific Advisor for the Horváth Csaba Memorial Laboratory lead by Prof. Andras Guttman. At the Horváth Csaba Memorial Laboratory Steve provides technical advice and supports the groups fundamental research and development of new applications in the area of CE and CE-MS.


    Abstract

    Despite recent developments in hyphenated techniques and column technology, analysis of small polar analytes (small organic acids, phosphorylated sugars, and underivatized amino acids) remains challenging.  These small charged metabolites are rarely retained on standard reverse phase columns and usually require HILIC/Hypercarb columns or ion chromatography.  In ion chromatography run times are usually long, Hypercarb columns usually need extensive conditioning before samples are run and to maintain peak shape HILIC chromatography often requires that extracts contains organic solvents such as acetonitrile which may not be the best solvent for highly polar compounds.  All of these techniques require different mobile phases or columns when you move between positive and negatively charged metabolites.

    In this webinar presentation, we present an alternative workflow, called CESI-MS, which not only overcomes these stationary-phase-based separation challenges, but is also capable of detecting these polar metabolites at physiological levels without the need for derivatization.  We will show how this technique can also be used to separate isobaric and structurally similar metabolites including phosphorylated sugars.



    Key Learning Points:



    • Fast, robust, and reliable method for high-quality metabolomic fingerprinting of both cationic and anionic metabolites without the need for derivatization.

    • How CESI-MS allows instant switching between negative and positive ionization modes without the need to change capillary or buffers.

    • Methods to separate isobaric and structurally similar metabolites based on their charge & hydrodynamic shape.


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