APR 27, 2021 8:00 AM PDT

Simplified Online Tools for CRISPR-Cas9 Gene Editing Design and Confirmation

Speaker

Abstract

Date:  April 27, 2021

Time: 08:00am (PDT)

 

The versatility of CRISPR-Cas9 has expanded the available toolset of gene editing systems for life scientists. However, these experiments require detailed knowledge of the genomic sequences, both from public reference databases as well as the editing outcomes. Obtaining proper bioinformatics support for either of these steps can be a challenge for both novices and experts. To this end, Thermo Fisher Scientific has built several software solutions to improve the usability of the CRISPR-Cas9 system by addressing two major challenges; the design of gRNAs and corresponding donor sequences, and confirmation of editing success at the sequence level through Sanger sequencing deconvolution.

This seminar will highlight our recent advancements in digital tools, including demonstrations of the Invitrogen TrueDesign Genome Editor online design tool and the Applied Biosystems SeqScreener Gene Edit Confirmation App.

 

Learning Objectives

  • Design reagents for a custom knockout or knock-in CRISPR-Cas9 experiment
  • Properly pair knock-in donor and gRNA design together
  • Import and analyze Sanger sequence reads for gene editing confirmation
  • Successfully infer outcomes from sequencing results and take appropriate next steps

 

Webinars will be available for unlimited on-demand viewing after live event.

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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