JUN 09, 2016 9:00 AM PDT

Simplifying your workflow with CellSimple™ Cell Analyzer: a complete solution for benchtop cellular analysis

Speakers
  • MD/PhD Student, Harvard University and MIT
    Biography
      Wilfredo F. Garcia-Beltran was born and raised in Puerto Rico, where he obtained a bachelor's degree in Chemistry in the University of Puerto Rico-Río Piedras Campus. After completing his undergraduate education, in 2010 he joined the Harvard/MIT MD/PhD program, where he is currently completing his PhD in immunology. His thesis research project focuses on the role of natural killer cells in HIV-1 infection, but through various collaborations, has also worked on further developing humanized mouse models and engineering novel anti-HIV-1 therapies.
    • Field Application Scientist, Cell Signaling Technology
      Biography
        Jonah Riddell received bachelors in chemistry and physics from Saint Bonaventure University in New York. Continued education included a master's degree in oncology from Roswell Park Cancer Institutes' department of biophysics/biochemistry where he was the first to crystalize the ologomeric form of Peroxiredoxin 1. During Ph.D. work in the RPCI division of the State University of NY at Buffalo program in immunology and cancer immunity he identified and characterized a novel prostate cancer antigen, now in clinical trials, that was found to play multiple roles in promoting prostate cancer. Post-doctoral completed at the Harvard Stem Cell Institute/Boston Children's Hospital succeeded in reprogramming murine differentiated blood cells into hematopoietic stem cells in work that hopefully can be pushed to the clinic and help patients undergoing bone marrow transplants. Peer reviewed research articles and two patents have shown his investment in clinically oriented therapies toward cancer. He continues his goals of aiding researchers by providing reliable and quality products from Cell Signaling Technology to researchers and clinicians so that millions of researchers can bear the torches to illuminate numerous novel disease therapies.

      Abstract

      DATE: June 9, 2016
      TIME:  9am Pacific time, 12pm Eastern time


      Complex cell and bead-based assays are simple and fast with the CellSimple™ Cell Analyzer. This instrument combines a 488 nm laser, dual photomultiplier tubes (PMT), Coulter Principle based cell measurements and on-board software to provide easy-to-run applications and data analysis. The instrument relies on disposable cassettes for sample handling, which alleviates the need for flow cell cleaning and fluidics maintenance. Plus, the instrument is small enough to be portable between the lab bench and the hood. When paired with highly validated kits and reagents from Cell Signaling Technology (CST), the CellSimple Cell Analyzer enables powerful plug-and-play assays, such as measuring cell health and apoptosis, or measuring signaling pathway activation.

      Wilfredo F. Garcia-Beltran is an MD/PhD student conducting his research in the laboratory of Marcus Altfield, MD, PhD at the Ragon Institute of MGH, MIT, and Harvard. The laboratory focuses on innate immune responses to HIV-1 infection. Mr. Garcia has worked extensively with the CellSimple Cell Analyzer to understand how it can be used to assess the absolute frequency of cell populations of interest, as well as identify cell subsets labeled with fluorophore-conjugated antibodies or transfected/transduced with eGFP-containing gene constructs. Within these workflows, he used the CellSimple Cell Analyzer to quickly asses eGFP expression from a number of immune and non-immune cells, quantify enrichment and cell health of sorted eGFP positive cells, phenotype immune cell populations based on surface marker expression, accurately determine cell counts of specific subpopulations (e.g., CD4+ T cell counts in human samples), and quantify viability of virtually any cell line or primary cell population.

      Learning Objectives:

      • To understand the functionality of CellSimple Cell Analyzer in a variety of workspaces
      • To observe real world examples of CellSimple Cell Analyzer use in research laboratories

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