NOV 10, 2016 10:00 AM PST

Single-cell analysis of virus infection: Zika & beyond

Sponsored by: Affymetrix, Affymetrix
Speaker
  • Assistant Professor, Department of Anesthesiology and Program in Immunology; University of Colorado
    Biography
      Dr. Eric T. Clambey received his PhD degree from Washington University in St. Louis. He is currently an Assistant Professor at University of Colorado School of Medicine, Department of Anesthesiology and Program in Immunology, and Director of the University of Colorado Cancer Center Flow Cytometry Shared Resource. His research program focuses on the dynamic interface between the immune system, infection, inflammation and tissue repair.

    Abstract
    DATE:  November 10, 2016
    TIME:  10am PT, 1pm ET

    How do you effectively detect heterogeneity in single cells?

    Bulk analysis often leads to conclusions that assume averages reflect the dominant biological mechanism operating within an entire population. To fully understand how cellular heterogeneity contributes to biological function, a single-cell analysis approach must be applied.

    Join us for a seminar on breakthroughs in single-cell gene expression research that have advanced our understanding of the immune response and are paving the way towards unmasking gene expression heterogeneity.

    The heterogeneity of RNA expression at the single-cell level is often obscured by conventional assays of pooled cellular material. Eric Clambey, PhD recently used PrimeFlow® RNA assay to detect virus and host RNA by flow cytometry, enabling single-cell analysis of virus-host dynamics. This methodology has been applied to detect Zika virus infection, simultaneously quantifying the plus-strand RNA genome and 3’ untranslated region. In parallel, this platform has been used to study herpesvirus-induced host shutoff, directly observing host mRNA degradation in virus-infected cells. These studies have revealed unanticipated heterogeneity in virus-infected cells, and identified new interrelationships between virus and host RNA at the single-cell level.

    Show Resources
    You May Also Like
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    APR 22, 2021 5:00 PM CEST
    APR 22, 2021 5:00 PM CEST
    Date: April 22, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) System transcriptomics of the schizophrenia brain - Phillip Khaitovich Schizophrenia tops the psychiatric disorder l...
    APR 21, 2021 5:00 PM CEST
    APR 21, 2021 5:00 PM CEST
    Date: April 21, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Spatial Answers Trilogy - Spatial Answers in Immunology Immunology Researchers share their Spatial Discoveries in SARS-C...
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    Loading Comments...
    Show Resources
    Attendees
    • See more