NOV 10, 2016 10:00 AM PST

Single-cell analysis of virus infection: Zika & beyond

SPONSORED BY: Affymetrix, Affymetrix
Speakers
  • Assistant Professor, Department of Anesthesiology and Program in Immunology; University of Colorado
    Biography
      Dr. Eric T. Clambey received his PhD degree from Washington University in St. Louis. He is currently an Assistant Professor at University of Colorado School of Medicine, Department of Anesthesiology and Program in Immunology, and Director of the University of Colorado Cancer Center Flow Cytometry Shared Resource. His research program focuses on the dynamic interface between the immune system, infection, inflammation and tissue repair.

    Abstract:
    DATE:  November 10, 2016
    TIME:  10am PT, 1pm ET

    How do you effectively detect heterogeneity in single cells?

    Bulk analysis often leads to conclusions that assume averages reflect the dominant biological mechanism operating within an entire population. To fully understand how cellular heterogeneity contributes to biological function, a single-cell analysis approach must be applied.

    Join us for a seminar on breakthroughs in single-cell gene expression research that have advanced our understanding of the immune response and are paving the way towards unmasking gene expression heterogeneity.

    The heterogeneity of RNA expression at the single-cell level is often obscured by conventional assays of pooled cellular material. Eric Clambey, PhD recently used PrimeFlow® RNA assay to detect virus and host RNA by flow cytometry, enabling single-cell analysis of virus-host dynamics. This methodology has been applied to detect Zika virus infection, simultaneously quantifying the plus-strand RNA genome and 3’ untranslated region. In parallel, this platform has been used to study herpesvirus-induced host shutoff, directly observing host mRNA degradation in virus-infected cells. These studies have revealed unanticipated heterogeneity in virus-infected cells, and identified new interrelationships between virus and host RNA at the single-cell level.

    Show Resources
    You May Also Like
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    JUN 05, 2019 05:00 PM CEST
    C.E. CREDITS
    JUN 05, 2019 05:00 PM CEST
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    SEP 05, 2019 04:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 04:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    MAY 21, 2019 07:00 AM PDT
    C.E. CREDITS
    MAY 21, 2019 07:00 AM PDT
    DATE: May 21, 2019TIMEL 7:00am PT, 10:00am ET Human T cells are central effectors of immunity and cancer immunotherapy. CRISPR-based functional studies in T cells could prioriti...
    NOV 18, 2019 07:00 AM PST
    C.E. CREDITS
    NOV 18, 2019 07:00 AM PST
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    JUN 26, 2019 09:00 AM PDT
    C.E. CREDITS
    JUN 26, 2019 09:00 AM PDT
    DATE: June 26, 2019TIME: 9:00am PDT, 12:00pm EDT An excessive number of software solutions are available to help manage your clinical, biobank, or biorepository sample inform...
    Loading Comments...
    Show Resources