OCT 08, 2020 9:00 AM PDT

The Spatiotemporal Organization, Dynamics, and Function of Virus Replication Centers during Murine Polyomavirus Infection

Sponsored by: Andor
C.E. Credits: Florida CE P.A.C.E. CE
Speakers
  • Postdoctoral Research Assistant - BioFrontiers Institute and the Department of Molecular, Cellular, and Developmental Biology (MCDB), University of Colorado Boulder
    BIOGRAPHY
  • Product Specialist for Microscopy Cameras at Andor Technology.
    BIOGRAPHY

Abstract
Date:  October 8, 2020
Time: 9:00am (PDT), 12:00pm (EDT), 5:00pm (GMT)
 
Small DNA viruses require both host DNA replication and repair factors for their replication. The host cell replication factors are often recruited by the virus to subnuclear domains referred to as viral replication centers (VRCs). Several factors have been immunolocalized to VRCs using fluorescence microscopy, but little else is known about VRC spatiotemporal organization or dynamics. We investigated the organization and function of VRCs during murine polyomavirus (MuPyV) infection using 3D structured illumination microscopy (3D-SIM). We immunolocalized viral and cellular VRC components to spatially distinct VRC subdomains through which viral DNA (vDNA) trafficked sequentially post-synthesis, suggesting these VRC subdomains have distinct functional roles in vDNA processing. We also observed the disruption of VRC organization and function during mutant MuPyV infections or inhibition of DNA synthesis. These results reveal a dynamic organization of VRC components that coordinates MuPyV replication during infection
 
Learning Objectives
  • This webinar will demonstrate how 3D-SIM can be used to study viral genome replication and the spatial organization of viral replication centers.
  • This webinar will address the application and limitations of quantitative image analysis for super-resolution microscopy data.
  • This webinar will discuss the use of complementary microscopy modalities for studying the polyomavirus-host interface.
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
JUL 15, 2021 9:00 AM PDT
JUL 15, 2021 9:00 AM PDT
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
Loading Comments...
Show Resources