OCT 08, 2020 9:00 AM PDT

The Spatiotemporal Organization, Dynamics, and Function of Virus Replication Centers during Murine Polyomavirus Infection

Sponsored by: Andor
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Postdoctoral Research Assistant - BioFrontiers Institute and the Department of Molecular, Cellular, and Developmental Biology (MCDB), University of Colorado Boulder
    Biography
      Douglas has a BA in Neuroscience and Biology from Augustana College in Rock Island, Illinois, and a PhD in MCDB from the University of Colorado Boulder.


       


      Douglas joined the Garcea Lab at the University of Colorado Boulder in 2014, where his thesis research used fluorescence microscopy and quantitative image analysis tools to study the spatiotemporal dynamics of polyomavirus replication during infection. Douglas defended his PhD in early 2020 and is currently continuing his research as a postdoctoral research assistant.
    • Product Specialist for Microscopy Cameras at Andor Technology.
      Biography
        My background in microscopy is built from my work and studies while at Queen's University Belfast. I always had a keen interest in bacteria and viruses as pathogens, as well as how we may exploit them for our benefit, so naturally specialised in microbiology. After my BSc (Hons) in 1999, I went on to do my PhD in microbiology within the School of Biology and Biochemistry at the Medical Biology Centre at Queens. My PhD was focused on polyphosphate metabolism of microorganisms- trying to get a better understanding of this molecule, how to quantify it, and the key roles this played within environmental and pathogenic bacteria. Roles include as a protective function in response to stress and in helping pathogens establish infections. An interesting application of this was in biological phosphate removal from wastewater for which we had a pilot scale study. This then led on to a Research Fellow position in the same research group of John Quinn and John McGrath in 2002. While in this position I worked further on connecting the biochemistry and genetics of polyphosphate metabolism. This included studies to characterise the bacterial transport systems involved and development of novel research tools, such as fluorescent assays to determine intracellular pH, and how to apply our luminescence and fluorescence imaging based methods to 96 well plate formats for faster screening and analysis. After Academia, I went on to work in the Medical Diagnostics and Pharmaceutical industries. I joined Andor Technology in 2012, initially working as a Technical Author for our camera and systems products. My current role is a Product Specialist for microscopy cameras. This role brings me in touch with the latest developments in camera technology, as well as a broad range of microscopy applications and techniques.

      Abstract
      Date:  October 8, 2020
      Time: 9:00am (PDT), 12:00pm (EDT), 5:00pm (GMT)
       
      Small DNA viruses require both host DNA replication and repair factors for their replication. The host cell replication factors are often recruited by the virus to subnuclear domains referred to as viral replication centers (VRCs). Several factors have been immunolocalized to VRCs using fluorescence microscopy, but little else is known about VRC spatiotemporal organization or dynamics. We investigated the organization and function of VRCs during murine polyomavirus (MuPyV) infection using 3D structured illumination microscopy (3D-SIM). We immunolocalized viral and cellular VRC components to spatially distinct VRC subdomains through which viral DNA (vDNA) trafficked sequentially post-synthesis, suggesting these VRC subdomains have distinct functional roles in vDNA processing. We also observed the disruption of VRC organization and function during mutant MuPyV infections or inhibition of DNA synthesis. These results reveal a dynamic organization of VRC components that coordinates MuPyV replication during infection
       
      Learning Objectives
      • This webinar will demonstrate how 3D-SIM can be used to study viral genome replication and the spatial organization of viral replication centers.
      • This webinar will address the application and limitations of quantitative image analysis for super-resolution microscopy data.
      • This webinar will discuss the use of complementary microscopy modalities for studying the polyomavirus-host interface.
       
       
      Webinars will be available for unlimited on-demand viewing after live event.
       
      LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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