JUN 09, 2016 9:00 AM PDT

Standardizing Microbiomics

Sponsored by: Zymo Research, Zymo Research
Speakers
  • Director of Nucleic Acid Solutions, Zymo Research Corp.
    Biography
      Ryan Kemp is currently the Director of Nucleic Acid Solutions for Zymo Research Corporation in Irvine, CA. Kemp joined Zymo in 2012 as a research scientist, and has since led the development of multiple novel DNA and RNA purification technologies. His background is in organic chemistry where he developed artificial receptors for anion recognition, based on the siderophore enterobactin. As the Director of Nucleic Acid Solutions, he been instrumental in development of many of Zymo Research's innovative products including ZymoPURE™ Plasmid DNA Kits, DNA/RNA Shield, and technologies inside many of the Quick-DNA™ kits. Kemp also contributed a textbook chapter in Sample Preparation Techniques for Soil, Plant, and Animal Samples (Springer, 2016) discussing techniques for nucleic acid isolation.
    • Scientist, Zymo Research Corp.
      Biography
        Dr. Shuiquan Tang is currently a research scientist at Zymo Research. He received his Ph.D. in the Department of Chemical Engineering and Applied Chemistry from the University of Toronto. Dr. Tang joined Zymo Research in 2014, and has since been instrumental in the research and development of Zymo's microbiomics and metagenomics programs. His past experiences include work in the fields of environmental microbiology and biological process engineering, with an emphasis on bioinformatics, metagenomics and anaerobic cultivation. Most recently, Dr. Tang has been involved in the development of Zymo Research's microbiomics portfolio. He has multiple publications in different journals, including Frontiers in Microbiology, Applied & Environmental Microbiology and Philosophical Transactions of the Royal Society of London, Series B, Biological Science.

      Abstract:
      DATE:  June 9, 2016
      TIME:   9AM Pacific time, 12pm Eastern time


      The rapid growth of Microbiomics has increased the demand for standard methods to improve the reproducibility and quality of the data being generated.   Therefore, there is a need for standard reference materials for the development, evaluation, calibration, and validation of complex microbiomics workflows encompassing sample collection, sample preparation, and analyses. Standardization of the following methods would greatly improve the quality of data generated:  (1) sample collection tools that can reliably provide a molecular snapshot at the time of collection by stabilizing the nucleic acids at ambient temperature and rendering the sample noninfectious for safe transport; (2) purification methodologies that take into consideration the biases associated with differential lysis efficiency of the organism being processed (e.g. gram-negative/positive bacteria, fungus, viruses, and associated spores);  (3)  analytical pipelines that reduce bias due to library preparation methods, PCR, and bioinformatics (e.g. GC content, log difference organism abundances, detection limit, and ability to distinguish closely related organisms).  To address these fundamental challenges, the scientists at Zymo Research have created reference materials for the development of the most accurate and unbiased workflows from sample collection to analyses. In this seminar, we will show how these reference materials improve the quality of data generated from Microbiomic studies.

      Learning objectives:
      1. Attendees will learn why there is a need for standard methods to improve data analysis within micriobiomics workflows.
      2. Attendees will learn how individual steps in their microbiomic workflow can be standardized to greatly improve the quality and reproducibility of the data generated.

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