MAR 05, 2019 9:00 AM PST

Strategies to normalize miRNA expression in serum and plasma.

Speakers
  • R&D Staff Scientist, Sample Preparation BioSciences, ThermoFisher Scientific, USA
    Biography
      Emily has worked as part of R&D at the Austin site for over 15 years. During her tenure, she has lead and contributed to multiple projects including sample preparation and analysis of nucleic acids (including real-time PCR) from a wide range of sample types, library preparation for sequencing, and purification and analysis of exosomes. Recently and for the past several years, her efforts have shifted to the development of high-throughput and automated DNA/RNA extraction methods from clinical samples (such as biofluids) for use within larger analysis workflows ranging from such as Pharmacogenomics, Pathogen/Microbial Detection, Disease studies and Biomarker studies.
    • Senior Product Application Scientist, Genetic Sciences Division - Thermo Fisher Scientific
      Biography

        Harita Veereshlingam is a Senior Product Application Scientist in the Genetic Sciences Division at Thermo Fisher Scientific. She has over 10 years of experience in product development with an emphasis on real time PCR assays for gene expression and miRNA analysis. In her current role, Harita is responsible for product portfolio growth through the development of novel applications, customer collaborations, technical notes, and webinars. She lives by and is a strong proponent of sustainable living. She has a PhD in Molecular Biology from the University of North Texas.


      Abstract
      DATE:  March 5, 2019
      TIME:  09:00am PST, 12:00pm EST
       
      MicroRNA(miRNA) are short non-coding single stranded RNA molecules that regulate gene expression at the post transcriptional level.  They are known to play a critical role in multiple biological processes including proliferation, differentiation, apoptosis and hematopoiesis. Dysregulation of miRNA expression therefore is associated with a number of pathological conditions such as inflammation, cardiovascular diseases, neurological disorders and several types of cancers. miRNAs are also found to be extremely stable in body fluids such as serum, plasma, urine, breast milk and saliva. Extracellular miRNAs are either packed into exosomes or loaded into high-density lipoproteins, or bound by AGO2 proteins outside on vesicles. These modes of action protect miRNAs from degradation and improve their stability. Because of their stability in body fluids, miRNAs are gaining importance as biomarkers in liquid biopsies. Real time qPCR technologies reliably and accurately detect and measure miRNAs levels from body fluids.  However, since no single miRNA is known to be constitutively expressed in body fluids, normalizing real time qPCR data for comparing miRNA expression levels in different samples continues to be a challenge. In this study, we demonstrate a systematic methodology to identify and verify stably expressed miRNAs in body fluids.  The stably expressed miRNAs are then used to normalize miRNA expression profiles in plasma.  We illustrate this workflow for identifying miRNA biomarkers in plasma using the TaqMan Human Advanced miRNA Panels on 96-well plates, Taqman Array Cards (TAC), and Open Array plates.  These different formats provide throughput flexibility for miRNA biomarker discovery research programs.
       
      Learning Objectives:
      • How to identify stably expressed/endogenous miRNAs in serum and plasma
      • How to use spike-in controls to normalize miRNA expression in difficult samples.
      • Workflow to identify normalizers in serum and plasma using TaqMan® Array Cards, Plates and OpenArray™

       

      LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

      Show Resources
      You May Also Like
      MAY 11, 2021 10:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      MAY 11, 2021 10:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
      JUN 09, 2021 7:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      JUN 09, 2021 7:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
      DEC 02, 2020 8:00 AM PST
      C.E. CREDITS
      DEC 02, 2020 8:00 AM PST
      DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
      NOV 16, 2020 8:00 AM PST
      C.E. CREDITS
      NOV 16, 2020 8:00 AM PST
      Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
      OCT 29, 2020 6:00 AM PDT
      C.E. CREDITS
      OCT 29, 2020 6:00 AM PDT
      Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
      DEC 15, 2020 10:00 AM PST
      C.E. CREDITS
      DEC 15, 2020 10:00 AM PST
      DATE: December 15, 2020 TIME: 10:00am PST Scientists from Thermo Fisher Scientific will walk us through the world of microorganisms. They will discuss their most recent research on viruses,...
      MAR 05, 2019 9:00 AM PST

      Strategies to normalize miRNA expression in serum and plasma.


      Specialty

      Molecular Biology

      Cancer Research

      Biomarkers

      Genetics

      Cell Biology

      Molecular Diagnostics

      Immunology

      Gene Expression

      Clinical Research

      Biotechnology

      Genomics

      Cancer Diagnostics

      Dna

      Cell Culture

      Laboratory Testing

      Geography

      North America48%

      Europe28%

      Asia15%

      Africa4%

      South America3%

      Oceania1%

      Registration Source

      Website Visitors43%

      Email Promotions42%

      WAVE Newsletter Promotion8%

      Social Media Promotions3%

      Trending Newsletter Promotion1%

      Other1%

      Job Title

      Student19%

      Research Scientist14%

      Scientist11%

      Educator/Faculty11%

      Medical Laboratory Technician8%

      Post Doc7%

      Executive7%

      Lab Management7%

      Facility/Department Manager6%

      Clinical Laboratory Scientist4%

      Medical Doctor/Specialist3%

      Biologist2%

      Marketing/Sales1%

      Chemist1%

      Organization

      Academic Institution35%

      Hospital13%

      Research Institute8%

      Clinical Laboratory8%

      Biotech Company8%

      Government4%

      Pharmaceutical Company3%

      Life Science Company3%

      Medical School2%

      Medical Center2%

      Non-Profit Organization1%

      Consultant1%

      Contract Research Organization (Cro)1%

      Industrial Company1%

      Other4%


      Show Resources
      Loading Comments...
      Show Resources
      Attendees
      • See more