APR 13, 2023 8:00 AM PDT

Novel subunit analysis for biotherapeutic development: increased coverage from a single injection

Sponsored by: SCIEX

Event Date & Time
Date:  April 13, 2023
Time: 8:00am (PDT),  11:00am (EDT), 5:00pm (CEST)
It is important to confirm the sequences and post-translational modifications (PTMs) of protein therapeutics to ensure drug safety and efficacy. Middle-down mass spectrometry (MS) combines the advantages of the bottom-up and top-down MS approaches by offering high sequence coverage of subunits following simple sample preparation. Traditionally, a middle-down workflow requires extensive method development and often involves multiple fragmentation techniques and/or injections to obtain high sequence coverage.
This webinar discusses a single injection workflow based on electron activated dissociation (EAD) that addresses these challenges in middle-down biotherapeutic characterization. It leverages the descriptive and reproducible EAD fragmentation provided by the ZenoTOF 7600 system with automated data analysis using Biologics Explorer software. As a result, the workflow significantly reduces the time and effort required for middle-down analysis of protein therapeutics, enabling routine middle-down analysis of monoclonal antibodies (mAbs) with consistently high sequence coverage.
Learning Objectives
  • Learn how the streamlined EAD-based middle-down workflow provides reproducible high sequence coverages of protein therapeutics in a single injection
  • Gain insights into the benefits of the EAD-based middle-down workflow for localization of PTMs, such as glycosylation and oxidation
  • Get to know the straightforward middle-down data analysis workflow provided by Biologics Explorer software
Webinars will be available for unlimited on-demand viewing after live event.

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