SEP 11, 2019 10:30 AM PDT

Structural analysis to understand the molecular mechanism for CPV species-jump

C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Associate Professor, Department of Biochemistry & Molecular Biology, Director of Cryo-EM Imaging Facility, Pennsylvania State University
    Biography
      Susan Hafenstein Is an Associate Professor in the Dept. of Biochemistry & Molecular Biology and the Director of Cryo-EM Imaging Facility at Pennsylvania State University. Her work focuses on using cryo EM to learn more about how viruses interact with the host.

    Abstract:

    Canine parvovirus (CPV) is an important pathogen causing severe diseases in dogs, including acute hemorrhagic enteritis, myocarditis and cerebellar disease. Cross-species transmission of CPV occurs as a result of mutations on the viral capsid surface that alter the species-specific binding to the host receptor, transferrin receptor type-1 (TfR). The interaction between CPV and TfR has been extensively studied and previous analyses have suggested the CPV-TfR complex is asymmetric. To enhance the understanding of the underlying molecular mechanisms, we determined the CPV-TfR interaction using cryo electron microscopy to solve the icosahedral (3.0 Å resolution) and asymmetric (5.0 Å resolution) complex structures. Structural analyses revealed conformational variations of the TfR molecules relative to the binding site, which translated into dynamic molecular interactions between CPV and TfR. For the first time, the precise footprint of the receptor on the virus capsid was identified along with the identity of the amino acid residues in the virus-receptor interface. Our ‘rock and roll’ model provides a novel explanation for previous findings and gives new insights into species jumping and the variation in host ranges associated with new pandemics in dogs.

    Learning Objectives:

    1) Virus binding to receptor is more dynamic than previously thought.
    2) Receptor binding causes changes to the overall virus capsid.


    Show Resources
    You May Also Like
    SEP 05, 2019 4:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 4:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    NOV 18, 2019 7:00 AM PST
    C.E. CREDITS
    NOV 18, 2019 7:00 AM PST
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    JUL 31, 2019 9:00 AM PDT
    C.E. CREDITS
    JUL 31, 2019 9:00 AM PDT
    DATE: July 31, 2019TIME: 9:00am PT, 12:00pm ET The choroid plexus, which makes up the blood-cerebrospinal fluid barrier in the central nervous system (CNS), lines the ventricle...
    AUG 15, 2019 9:00 AM PDT
    C.E. CREDITS
    AUG 15, 2019 9:00 AM PDT
    DATE: August 15, 2019TIME: 9:00am PDT, 12:00pm EDT Radiation therapy is a critical tool for the treatment of brain tumors, however, exposure to high doses of ionizing radiation...
    DEC 05, 2019 9:00 AM PST
    C.E. CREDITS
    DEC 05, 2019 9:00 AM PST
    DATE: December 5, 2019TIME: 9:00am PST, 12:00pm EST Characterization of the spatial distribution and abundance of proteins and mRNAs with morphological context within tissues e...
    AUG 13, 2019 9:00 AM PDT
    C.E. CREDITS
    AUG 13, 2019 9:00 AM PDT
    DATE: August 13, 2019TIME: 9:00am PT, 12:00pm ET, 5:00pm BST Molecular complexes are major constituents of cells, hence unraveling their mechanisms is key to fuller comprehension of c...
    Loading Comments...
    Show Resources
    Attendees