SEP 11, 2019 10:30 AM PDT

Structural analysis to understand the molecular mechanism for CPV species-jump

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Associate Professor, Department of Biochemistry & Molecular Biology, Director of Cryo-EM Imaging Facility, Pennsylvania State University
    Biography
      Susan Hafenstein Is an Associate Professor in the Dept. of Biochemistry & Molecular Biology and the Director of Cryo-EM Imaging Facility at Pennsylvania State University. Her work focuses on using cryo EM to learn more about how viruses interact with the host.

    Abstract

    Canine parvovirus (CPV) is an important pathogen causing severe diseases in dogs, including acute hemorrhagic enteritis, myocarditis and cerebellar disease. Cross-species transmission of CPV occurs as a result of mutations on the viral capsid surface that alter the species-specific binding to the host receptor, transferrin receptor type-1 (TfR). The interaction between CPV and TfR has been extensively studied and previous analyses have suggested the CPV-TfR complex is asymmetric. To enhance the understanding of the underlying molecular mechanisms, we determined the CPV-TfR interaction using cryo electron microscopy to solve the icosahedral (3.0 Å resolution) and asymmetric (5.0 Å resolution) complex structures. Structural analyses revealed conformational variations of the TfR molecules relative to the binding site, which translated into dynamic molecular interactions between CPV and TfR. For the first time, the precise footprint of the receptor on the virus capsid was identified along with the identity of the amino acid residues in the virus-receptor interface. Our ‘rock and roll’ model provides a novel explanation for previous findings and gives new insights into species jumping and the variation in host ranges associated with new pandemics in dogs.

    Learning Objectives:

    1) Virus binding to receptor is more dynamic than previously thought.
    2) Receptor binding causes changes to the overall virus capsid.


    Show Resources
    You May Also Like
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    OCT 08, 2020 7:00 AM PDT
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    AUG 25, 2020 8:00 AM PDT
    C.E. CREDITS
    AUG 25, 2020 8:00 AM PDT
    DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    DEC 16, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 16, 2020 8:00 AM PST
    Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
    DEC 17, 2020 9:00 AM PST
    C.E. CREDITS
    DEC 17, 2020 9:00 AM PST
    Date: December 17, 2020 Time: 9:00am (PST), 12:00pm (EST) Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging moda...
    SEP 11, 2019 10:30 AM PDT

    Structural analysis to understand the molecular mechanism for CPV species-jump

    C.E. Credits: P.A.C.E. CE Florida CE

    Specialty

    Infectious Disease

    Pcr/rt-Pcr/real-Time Pcr

    Dna

    Gene Expression

    Dna Sequencing

    Biomarkers

    Molecular Biology

    Laboratory Testing

    Protein

    Immunology

    Genetics

    Biotechnology

    Neuroscience

    Microbiology

    Antibodies

    Geography

    Asia57%

    Europe29%

    North America14%

    Registration Source

    Website Visitors100%

    Job Title

    Student33%

    Educator/Faculty33%

    Microbiologist17%

    Research Scientist17%

    Organization

    Academic Institution57%

    Government29%

    Pharmaceutical Company14%


    Show Resources
    Loading Comments...
    Show Resources
    Attendees
    • See more