JAN 29, 2020 9:00 AM PST

A case study of protein sample prep strategies for membrane proteins

Speaker
  • Senior R&D Manager, Protein Biology, Thermo Fisher Scientific
    Biography
      Barbara Kaboord received a Ph.D. in Biochemistry at the Medical College of Wisconsin, where she developed a method to affinity isolate DNA replication complexes from actively-replicating cells and followed up with a postdoctoral fellowship at the Pennsylvania State University in Stephen Benkovic's laboratory. Currently, Barbara is the Sr. R&D Manager of the Protein Preparation group at Thermo Fisher Scientific where her team is responsible for the development of numerous protein extraction, stabilization, purification, and clean-up products.

    Abstract

    DATE: January 29, 2019
    TIME: 9:00am PST

     

    Plasma membrane proteins are a difficult subset of proteins to solubilize, purify, quantify, and detect, largely due to the challenge of extracting them out of their native lipid environment, keeping them soluble, and the shortage of good antibodies.  Commonly used detergents may effectively solubilize these proteins but do not necessarily preserve their function or native structure and may interfere in downstream applications.  

    This webinar will focus on different membrane protein solubilization strategies and what enrichment or purification is necessary depending upon the downstream mode of detection or analysis.

     

    Learning Objectives:

    • Describe factors to consider when expressing and purifying recombinant proteins
    • Describe methods to solubilize membrane proteins
    • Choose options for effectively stabilizing GPCR activity from extraction through purification and clean-up

     

    Webinars will be available for unlimited on-demand viewing after live event.

     

    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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