Speaker: SureQuant pTyr - a platform for targeted profiling of tyrosine phosphorylation signaling dynamics in human tumors

Tyrosine phosphorylation (pTyr) plays a pivotal role in signal transduction and is commonly dysregulated in cancer, however existing MS methods to profile pTyr require a comprise between broad coverage, reproducibility, and quantitively accuracy. As a result, we developed “SureQuant pTyr,” a targeted approach coupling low input pTyr enrichment with a panel of isotopically labeled, tyrosine phosphorylated internal standard (IS) peptides for IS-guided acquisition using the SureQuant platform. We compare SureQuant pTyr to traditional acquisition approaches and demonstrate the method’s clinical utility in human colorectal tumors.