MAY 11, 2016 06:00 AM PDT

Expanding the molecular toolbox for Nanopore sequencing

  • Associate Director of Applications, Oxford Nanopore Technologies
      Having completed my undergraduate degree in 2007 (Wadham College, Oxford) I got the taste for nanopore technology during a brief stint at ONT that Summer. Subsequently, I went to work for Hagan Bayley, obtaining my DPhil, before re-joining the company in 2011 focussing on library preparation development.


    Library preparation for Nanopore sequencing is a very flexible process. There are many ways in which a sequencing template, or other analyte, can be presented to a nanopore for analysis. PCR may or may not be performed, shearing or size selection is not necessary but can be helpful in specific circumstances. You can sequence both strands in a duplex, or just one ... and what’s more, the platform is not limited to just sequencing DNA, but also RNA, and has been used to sense proteins and a wide variety of small molecules. The versatility of the system gives users access to a wide variety of library preparation options, depending on the needs of their experiments: ultra-long reads (>50 kb), low inputs (<50 ng), rapid and simple preps (10 minutes). In this presentation, I discuss the myriad options that are available and enabled with Nanopore sequencing.

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