Library preparation for Nanopore sequencing is a very flexible process. There are many ways in which a sequencing template, or other analyte, can be presented to a nanopore for analysis. PCR may or may not be performed, shearing or size selection is not necessary but can be helpful in specific circumstances. You can sequence both strands in a duplex, or just one ... and what’s more, the platform is not limited to just sequencing DNA, but also RNA, and has been used to sense proteins and a wide variety of small molecules. The versatility of the system gives users access to a wide variety of library preparation options, depending on the needs of their experiments: ultra-long reads (>50 kb), low inputs (<50 ng), rapid and simple preps (10 minutes). In this presentation, I discuss the myriad options that are available and enabled with Nanopore sequencing.
Medical Laboratory Technician33%
Manufacturer - Other33%