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Thermo Scientific Cell Locker System Segregates Stem Cells, Protecting from Contamination and Enhancing Environmental Stability

Speaker
  • Senior Global Cell Culture Scientist, Thermo Fisher Scientific
    Biography
      Mary Kay Bates is a Senior Global Cell Culture Scientist with Thermo Fisher Scientific, where she provides cell culture expertise to colleagues and customers. Her knowledge is based on twenty years of experience in academic and industrial cell and molecular biology labs, focusing on cancer and gene therapy, authoring several publications. Mary Kay holds an M.S. in microbiology from the University of Wisconsin-Madison, and has presented seminars at institutes around the world.

    Abstract

    Delicate cultures including stem cells and primary cells are increasingly used in cell therapy research and development for applications in oncology, immunology, neurology and more. Commonly, these cells require daily manipulation, potentially exposing them to contamination. Such cells are also uniquely reactive to environmental variation. To address these challenges, we developed the Thermo Scientific™ Cell Locker™ System, a new CO2 incubator design which includes six individual transparent Cell Locker chambers. Tests show the Cell Locker System provides enhanced stability, contamination control and flexibility for isolation of labile cell types or sensitive projects in a shared incubator. Each Cell Locker chamber features dual 0.2 µm membrane filters which allow air and humidity exchange but prevent transmission of microorganisms including mycoplasmas.

    Functional studies show that when one Cell Locker chamber is opened, remaining chambers in the Cell Locker System provide environmental stability for temperature, humidity, and CO2/O2 atmosphere, maintaining ideal conditions not possible in a standard incubator. Gas use and media evaporation were both reduced by 50%. Multiple clones of induced pluripotent stem cells from different donors were evaluated and found to be indistinguishable from control incubators where conditions and manipulations had previously been optimized, showing that the Cell Locker System design and materials facilitated optimal cell growth. The Cell Locker System represents a novel approach for culturing stem cells and other sensitive cultures used in cell therapy applications.


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