JAN 31, 2023 10:00 AM PST

Tips and tricks for successful multiplexing IHC

Speaker

Abstract
Date:  January 31, 2023
Time: 10:00am (PST),  1:00pm (EST), 7:00pm (CEST)
 
Immunohistochemistry (IHC) is a standard technique for scientists and pathologists to detect antigens. Examining one or two markers is a straightforward process but the level of complexity increases as more markers are added in complex samples (e.g., tumor microenvironments). Expanding to include more markers in an IHC experiment, requires in-depth knowledge about sample preparation, fluorescent dyes, and microscopes. This webinar will elucidate how to select markers and fluorophore dyes to a simple IHC experiment or start a larger Spatial Imaging panel. We will discuss how to prevent sample autofluorescence, unspecific antibody labeling, fluorescent bleed-through and spectral overlap.
 
Learning Objectives
  • Discover easy-to-incorporate techniques and tips to prevent nonspecific background effects or inadequate detection by dyes
  • Review basic methods from embedding, blocking, antibody labeling, mounting, and imaging the sample
  • Investigate free webtools to help design IHC experiments incorporating multiple fluorescent dyes
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
Labroots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

Show Resources
You May Also Like
FEB 15, 2023 7:00 AM PST
FEB 15, 2023 7:00 AM PST
Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
NOV 16, 2022 7:00 PM PST
C.E. CREDITS
NOV 16, 2022 7:00 PM PST
Date: November 16, 2022 Time: 2:00pm (AEST) Date: November 17, 2022 7:00pm (PST), 10:00pm (EST), 4:00am (CET) The growth in FDA-approved cell and gene therapy products for the treatment of d...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
OCT 13, 2022 9:00 AM +08
OCT 13, 2022 9:00 AM +08
First Broadcast: Date: October 12, 2022 Time: 8:00am PDT, 11:00am EDT Second Broadcast: Date: October 12, 2022 Time: 9:00am SGT The new Embedded CryoSPARC Live, now fully integrated with t...
NOV 09, 2022 8:00 AM PST
C.E. CREDITS
NOV 09, 2022 8:00 AM PST
Date: November 09, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) The field of cell and gene therapy is rapidly growing. In particular, the use of lentiviruses in CAR-T applications is...
AUG 10, 2022 10:00 AM PDT
AUG 10, 2022 10:00 AM PDT
Date: August 10, 2022 Time: 10:00am PDT, 1:00pm EDT The global pandemic has increased focus and scrutiny on molecular diagnostic assay development, resulting in a need for assays that provid...
Loading Comments...
Show Resources
Event Countdown
  • 0 Days
  • 0 Hours
  • 0 Minutes
  • 0 Seconds