JAN 31, 2023 10:00 AM PST

Tips and tricks for successful multiplexing IHC


Event Date & Time
Date:  January 31, 2023
Time: 10:00am (PST),  1:00pm (EST), 7:00pm (CEST)
Immunohistochemistry (IHC) is a standard technique for scientists and pathologists to detect antigens. Examining one or two markers is a straightforward process but the level of complexity increases as more markers are added in complex samples (e.g., tumor microenvironments). Expanding to include more markers in an IHC experiment, requires in-depth knowledge about sample preparation, fluorescent dyes, and microscopes. This webinar will elucidate how to select markers and fluorophore dyes to a simple IHC experiment or start a larger Spatial Imaging panel. We will discuss how to prevent sample autofluorescence, unspecific antibody labeling, fluorescent bleed-through and spectral overlap.
Learning Objectives
  • Discover easy-to-incorporate techniques and tips to prevent nonspecific background effects or inadequate detection by dyes
  • Review basic methods from embedding, blocking, antibody labeling, mounting, and imaging the sample
  • Investigate free webtools to help design IHC experiments incorporating multiple fluorescent dyes
Webinars will be available for unlimited on-demand viewing after live event.
Labroots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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