Scientist, R&D, Protein and Cell Analysis, Thermo Fisher Scientific
BIOGRAPHY
JUN 20, 2023 10:00 AM PDT
Tissue preparations and handling for multiplex imaging in spatial biology research
Speaker
Event Date & Time
Date: June 20, 2023
Time: 10:00 AM (PDT), 1:00 PM (EDT)
Abstract
Spatial biology enables researchers to characterize combined molecular information and spatial resolution in context. By using this technique on tissues, researchers can apply multiple different detection modalities and elucidate the spatial relationship among individual cell types & tissue niches. Due to the complexity of different tissue types and the added complication of different preservation methods( ie Fresh frozen (FF), formalin-fixed, or formalin-fixed paraffin embedded (FFPE) ), initial experimental conditions must be optimized and taken into consideration when studying multiple proteins and RNA targets. These include (1) tissue handling and fixation (2) epitope accessibility (3) potential for autofluorescence (4) mounting needs. In this webinar we provide guidance, key considerations for obtaining spatial biology data.
Learning Objectives
- Describe tissue preparation methods for multiplex antibody staining
- Discover appropriate controls for complex staining experiments
- Master epitope retrieval methods, blocking, and minimizing autofluorescence
Webinars will be available for unlimited on-demand viewing after live event.
Labroots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.
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