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NOV 16, 2021 10:00 AM PST

Towards a mechanistic understanding of motile and primary cilia with CLEM and cryo-electron tomography and expansion microscopy

Speaker

Abstract
Date: November 16, 2021
Time: 10:00am PST, 1:00pm EST
 
In this talk I will show how the power of modern electron microscopy (EM) techniques, coupled with light microscopy and expansion methods, can open a window to the molecular dynamics of cells, enabling us to discover details about cellular processes at unprecedented level of detail. More concretely, I will show how we have used time resolved correlative light and electron microscopy (CLEM), cryo-FIB-SEM/cryo-electron tomography (cryo-ET) and expansion microscopy (U-ExM) paired with confocal fluorescence microscopy, TIRF microscopy and mechanical manipulations of cells to study a number of essential molecular processes in motile and primary cilia, i.e. conserved organelles that are fundamental for most eukaryotic cells. While our own work focuses on the inner workings of cilia, many open questions in cell and molecular biology can be addressed by combining modern imaging methods and methods from cell biology in suitable ways.
 
 
Learning Objectives:
  • Discuss how cryo-electron tomography (cryo-ET) is used for imaging the interior of cells at high resolution
  • Describe how cryo-ET can be combined with cell biology methods thru correlative light electron microscopy
  • Discuss new understanding of the processes involved in assembly and function of motile and primary cilia, conserved organelles that are fundamental for most eukaryotic cells
 
 
 

Webinars will be available for unlimited on-demand viewing after live event.

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

 


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