SEP 12, 2018 12:00 PM PDT

Tracking Antibiotic Resistance Across Space and Time

Speaker

Abstract

Horizontal gene transfer (HGT) has long been recognized as an important source of genetic diversity. In the clinical setting, HGT plays an important role in the spread of antibiotic resistance genes and has the ability to turn easily treatable infections by common organisms like E. coli into untreatable, life-threatening conditions. Carbapenem-resistance, often mediated by plasmids carrying the blaKPC gene, is a worldwide threat. Although evidence-based guidelines to prevent transmission of carbapenemase-producing organisms (CPO) exist, they don't explicitly take into account HGT; which may complicate tracking nosocomial transmission. Here I'll present clinical investigations that underscore the consequences of HGT.

First, I'll present a study that tracked the long-term colonization of patients from a 2011 K. pneumoniae outbreak have remained consistently colonized with CPOs. Analysis of isolates collected from these patients revealed a complex network of plasmids, plasmid rearrangements and evidence of strain succession and re-emergence. Then I'll describe a study conducted at our hospital, where broad-range environmental sampling was conducted, including housekeeping closets, wastewater from hospital internal pipes, and external manholes. Whole-genome sequencing and analysis of 108 carbapenem-resistant isolates was compared to five years of patient isolates. We identified a vast, underappreciated reservoir of CPOs and identified a number of putative transmission links between patients and the environment. 

Learning Objectives: 

1. Understand the importance of horizontal gene transfer for the spread of antibiotic resistance
2. Appreciate the diversity of antibiotic resistance plasmids in environmental reserviors


Show Resources
You May Also Like
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
AUG 09, 2022 11:00 AM PDT
AUG 09, 2022 11:00 AM PDT
Date: August 9, 2022 Time: 11:00am (PST), 2:00pm (EST), 8:00pm (CEST) Antimicrobial resistance (AMR) has emerged as a major health threat. The FED-AMR project part of the One Health European...
C.E. CREDITS
Date: October 25, 2022 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CEST) Candida auris is a multi-drug resistant yeast that continues to be a global threat for infection and transmission in h...
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
NOV 16, 2022 7:00 PM PST
C.E. CREDITS
NOV 16, 2022 7:00 PM PST
Date: November 16, 2022 Time: 2:00pm (AEST) Date: November 17, 2022 7:00pm (PST), 10:00pm (EST), 4:00am (CET) The growth in FDA-approved cell and gene therapy products for the treatment of d...
OCT 12, 2022 9:00 AM PDT
C.E. CREDITS
OCT 12, 2022 9:00 AM PDT
Date: October 12, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) The microbiome of every food product is incredibly diverse and can include everything from innocuous background flora...
SEP 12, 2018 12:00 PM PDT

Tracking Antibiotic Resistance Across Space and Time



Show Resources
Loading Comments...
Show Resources