SEP 26, 2018 1:30 PM PDT

Two-Dimensional and Three-Dimensional Cell Culture, Does Dimension Really Matter?

Presented at: Cell Biology 2018
Speaker
  • Post-Doctoral Research Fellow at Phamacen North-West University (NWU)
    Biography
      I completed my B.Sc in Biological Sciences at the North-West University (NWU) in 2010, followed by a Hons. B.Sc in Biochemistry (NWU) in 2011. In 2012 I accepted a position as senior laboratory technician in the department Pharmaceutics at NWU. This enabled me to pursue a M.Sc in Pharmaceutics (2013-2014). In 2014 I was invited to join the international Golden Key honour society for my academic achievements as part of the top 15% of students in my field at NWU.

      I completed my Ph.D in Pharmaceutics in 2017 under the guidance of Dr C. Gouws, Dr K. Wrzesinksi and Prof S.Hamman. My thesis focused on the establishment of three-dimensional cell culture models for drug bio-transformation and toxicity studies. I was awarded the South African National Research Foundation (NRF) Innovation doctoral scholarship and travel award in 2016 and 2017. With this award I was able to spend six months at the University of Southern Denmark (SDU) in Odense, Denmark, learning the art of three-dimensional spheroid culturing.
      Since 2014 I have published five first author publications in international peer-reviewed journals. In 2014 I presented at the Young Scientist award of the 35th conference of the Academy of Pharmaceutical Sciences of South Africa. I have also acted as reviewer for the journal Current Pharmacology Reports, and hold membership to the South African Academy of Pharmaceutical Sciences.

      Currently I am employed in the faculty of Health Sciences at the NWU as a post-doctoral research fellow, focusing on the development of three-dimensional cell culture models and platforms for cancer research, bio-transformation and toxicity screening of pre-clinical lead compounds or traditional herbal medicine.

    Abstract

    When looking to answer complex biological questions, using in vitro techniques, you want the model that best reflects the in vivo condition.  For decades two-dimensional cell culture has dominated in this regard.  However, is it accurate to equate flat two-dimensional growing single monolayer cells to a complex three-dimensional tissue or organ system? Organs boast a unique three-dimensional cellular architecture, with cell-cell and cell-matrix interactions, creating a complex communication network through biochemical and mechanical signals.  However, important and distinct differences exist between 2D and 3D cell culturing, as well as the in vivo situation.  Differences between these two culturing extremes culminate into discrepancies in treatment responses.  Proof of concept suggests that 3D models may be more apt at providing information when evaluating new lead compounds.  Various types of 3D cell culture model systems are currently available and being explored.  The choice of system depends on the hypothesis, study design or target organ, and not one system is superior to the other with each offering various advantages and disadvantages.  The dynamic micro-gravity spheroid 3D system exhibits the ability to overcome many of the shortcomings of traditional 2D cell cultures.  In implementing this system in our laboratories, findings obtained from 2D and 3D in vitro hepatoxicity experiments showed significant discrepancies between the two systems.  While 3D experiments compared to an in vivo animal model, indicated that the 3D model is able to accurately predict possible hepatoxic events.  Looking at all the information provided, I put it to you, does dimension really matter?

    Learning Objectives: 

    1. What are the major differences between two-dimensional and three-dimensional cell culture, and how will this influence results.
    2. The potential applications of novel three-dimensional cell culture models and techniques in cell biology.


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