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SEP 17, 2020 4:30 PM PDT

Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Chief Commercial Officer, Molecular Biology Systems, BV
    Biography

      Brian McNally joined Molecular Biology Systems (MBS) as Chief Commercial Officer after 12 years in the life science and diagnostic industry. Previously, Brian has commercialized numerous nucleic acid, protein and cell-based assays for leading technology providers, many focused on multiplex panels for infectious and genetic diseases. Brian has fifteen years of practical experience at the laboratory bench, and his work has been published and presented at international conferences. Brian has a BSC from Carnegie Mellon University and a doctorate in Cell & Molecular Biology from University of Texas Southwestern.


    Abstract

    The COVID-19 pandemic has resulted in demand for population scale pathogen screening. The most sensitive and reliable method for detecting SARS-CoV-2 remains reverse transcription PCR (RT-PCR). However, current platforms for SARS-CoV-2 RNA detection have not yet met the demand for large scale, rapid testing. Here, we present an ultra-fast PCR platform, NEXTGENPCR, which accelerates RT-PCR assays. The NEXTGENPCR machine provides a small footprint capable of running PCR in 96 or 384 well plates in less than 10 minutes; and RT-PCR in less than 16 minutes. Come learn how you can accelerate RT-PCR for direct detection or sequencing applications.

    Learning Objectives:

    1. Understand how NEXTGENPCR accelerates SARS-CoV-2 assays and chemistries

    2. Considerations for setting up an efficient workflow to process >1,000 per hour

    3. Understand how accelerated cycling yields equivalent assay performance


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