SEP 17, 2020 4:30 PM PDT

Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2

C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract

The COVID-19 pandemic has resulted in demand for population scale pathogen screening. The most sensitive and reliable method for detecting SARS-CoV-2 remains reverse transcription PCR (RT-PCR). However, current platforms for SARS-CoV-2 RNA detection have not yet met the demand for large scale, rapid testing. Here, we present an ultra-fast PCR platform, NEXTGENPCR, which accelerates RT-PCR assays. The NEXTGENPCR machine provides a small footprint capable of running PCR in 96 or 384 well plates in less than 10 minutes; and RT-PCR in less than 16 minutes. Come learn how you can accelerate RT-PCR for direct detection or sequencing applications.

Learning Objectives:

1. Understand how NEXTGENPCR accelerates SARS-CoV-2 assays and chemistries

2. Considerations for setting up an efficient workflow to process >1,000 per hour

3. Understand how accelerated cycling yields equivalent assay performance


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
NOV 30, 2021 10:00 AM PST
C.E. CREDITS
NOV 30, 2021 10:00 AM PST
Date: November 30, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The prevalence of thyroid disease worldwide has served as a catalyst for healthcare providers to study various tools and methods to...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
OCT 27, 2021 6:00 AM PDT
C.E. CREDITS
OCT 27, 2021 6:00 AM PDT
Date: October 27, 2021 Time: 6:00 AM PDT, 9:00 AM EDT Etanercept is a recombinant Fc fusion protein therapeutic that has a complex distribution of post-translation modifications (PTM), such...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
Loading Comments...
Show Resources