Department of Inflammation and Ageing, Birmingham Biomedical Research Centre, University of Birmingham.
BIOGRAPHY
SEP 25, 2025 7:00 AM PDT
Ultracentrifugation Meets Nano-Flow Cytometry: A Practical Workflow for Extracellular Vesicle Enumeration and Integrity Assessment
Speaker
Event Date & Time
Date: September 25, 2025
Time: 7:00 AM (PDT), 10:00 AM (EDT), 4:00 PM (CEST)
Abstract
Ultracentrifugation (UC) remains a widely used method for isolating extracellular vesicles (EVs), but debates persist around yield, integrity, and optimal protocols. In this webinar, I will provide a practical overview of combining UC with nano-flow cytometry (CytoFLEX nano) for EV analysis. Drawing from current literature and in-house data, I will explore how spin time impacts pellet and supernatant yield, including a case study investigating EV differences in obesity. Calcein staining results suggest vesicle integrity is maintained post-UC. Attendees will gain insights into applying and evaluating this workflow in their own EV research.
Learning Objectives
- Understand how to combine ultracentrifugation and nano-flow cytometry for reproducible EV enumeration.
- Understand the importance of verifying EV integrity post-isolation, and how calcein-based staining can support this.
- Evaluate how ultracentrifugation spin time affects EV recovery from pellet vs. supernatant, and how this may vary depending on the biofluid being studied.
For Research Use Only. Not for use in diagnostic procedures.
Webinars will be available for unlimited on-demand viewing after live event.
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