OCT 05, 2016 6:00 AM PDT

Unique molecular indices (UMI) and their application in detecting novel gene fusions and gene expression and genetic variation

Speaker

Abstract

Tumor heterogeneity has been known for a while but quantifying heterogeneity is still a challenge.  NGS is the method of choice in the analysis of tumor heterogeneity, however, there are some inherent challenges associated with it. These include false positives, gaps in the gene due to overrepresentation and incomplete representation of low-frequency transcripts – all contributing to an inaccurate picture.  Conventional library prep strategies for NGS are based on PCR, which introduces sequence-based bias and amplification noise, leading to these inaccuracies. 

In this webinar, we will cover

  1. Principles of UMI and the new QIAseq product porfolio
  2. How UMI along with SPE (single primer extension) allows for increased uniformity across difficult-to-sequence regions, removal of library construction bias, improved data analysis and sequencing optimization
  3. How data generated from using UMI and SPE is directly comparable to analysis derived from whole transcriptome and exome sequencing
  4. Application of UMI and SPE in the discovery of novel gene fusions and in the analysis of gene expression and genetic variation

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OCT 05, 2016 6:00 AM PDT

Unique molecular indices (UMI) and their application in detecting novel gene fusions and gene expression and genetic variation


Specialty

Cancer

Research

Personalized Medicine

Gene Expression

University

Dna

Mass Cytometry

Cancer Research

T-Cells

Biomarkers

Immunotherapeutics

Oncology

Molecular Genetics

Gene Sequencing

Health

Geography

Europe75%

Asia25%

Registration Source

Website Visitors100%

Job Title

Student33%

Clinical Laboratory Scientist33%

Medical Laboratory Technician33%

Organization

Academic Institution50%

Hospital25%

Manufacturer - Other25%


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