OCT 17, 2013 9:00 AM PDT

Using DNASTAR Software to Identify Cancer Variations in Targeted Resequencing Data

Speaker

Abstract

DNASTAR offers an integrated suite of software for assembling and analyzing data from all major next-generation sequencing platforms. The software supports a variety of reference guided and de novo assembly workflows, including whole genomes, transcriptomes, targeted resequencing, ChIP-Seq and miRNA. In this presentation, we will show two different assembly projects that can be completed on a desktop computer using DNASTAR software: 1) bacterial genome assembly with gap closure using a reference genome from a related organism and 2) alignment of multiple human exomes against a reference genome that includes GenBank, dbSNP, Cosmic, and GERP annotations. After each assembly, the software can be used to view depth of coverage, read alignment, and SNPs and indels. SNPs can be filtered based on a variety of factors, including impact on gene function, phenotypic and ontology data for associated genes, and presence/absence in dbSNP and other databases. In the case of multiple samples, SNPs can also be compared across samples or groups. The integration of all these tools into one software package facilitates fast, comprehensive analysis, helping scientists move quickly from raw next-gen sequencing data to meaningful results. By using innovative algorithms within the software, scientists can have all of the assembly and analysis capabilities available to them on their desktop computer. Learner Objectives: Have a better understanding of sequence assembly and analysis software More familiarity with large-scale targeted re-sequencing (exome) assembly and analysis More familiarity with RNA-Seq expression analysis


Show Resources
You May Also Like
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
DEC 01, 2021 7:00 AM PST
C.E. CREDITS
DEC 01, 2021 7:00 AM PST
Date: December 01, 2021 Time: 7:00am (PST), 10:00am (EST) In the era of immuno-oncology, there is a growing need for the identification of new biomarkers predictive for sensitivity to anti-P...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
DEC 09, 2021 11:00 AM PST
C.E. CREDITS
DEC 09, 2021 11:00 AM PST
Date: December 09, 2021 Time: 11:00am (PDT), 2:00pm (EDT) The burden of antimicrobial resistance (AMR) has been acknowledged worldwide by leading health institutes. Besides the need for new...
SEP 16, 2021 8:00 AM PDT
C.E. CREDITS
SEP 16, 2021 8:00 AM PDT
Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
OCT 17, 2013 9:00 AM PDT

Using DNASTAR Software to Identify Cancer Variations in Targeted Resequencing Data



Show Resources
Loading Comments...
Show Resources