MAR 24, 2021 8:00 AM PDT

Using iPS-derived cells to generate a functional human liver through 3D bioprinting

Speaker
  • Post-doctoral Research Fellow
    Biography

      Post-Doctoral Research Fellow at the Human Genome and Stem Cell Research Center at the University of Sao Paulo - Brazil. Stem cell and Bioengineering scientist with 13 years' experience in biomedical research. Working to generate new technology to mitigate the scarcity of viable organs for transplantation. Author of several patents and high impact papers (e.g. Nat. Comm, Biofabrication, Cancer Research, etc). Expert in iPS cell technology and tissue engineering (3D bioprinting, organoids, etc). Passionate and dedicated scientist. Currently working with business development, gene-editing technology, xenotransplantation, liver organoid, and bioprinting.


    Abstract

    Date:  March 24, 2021

    Time: 08:00am PDT

     

    The liver is responsible for many metabolic, endocrine and exocrine functions. Approximately 2 million deaths per year are associated with liver failure. Modern 3D bioprinting technologies allied with autologous induced pluripotent stem cells (iPS)-derived grafts could represent a relevant tissue engineering approach to treat end stage liver disease patients. In this presentation, we will show how to differentiate human iPS towards relevant liver cellular phenotypes (hepatocytes, endothelial cells and mesenchymal cells) for 3D bioprinting. Also, we will evaluate the impacts of using single cell dispersion (i.e. obtained from conventional bidimensional differentiation) of iPS-derived parenchymal (i.e. hepatocyte-like cells) versus using iPS-derived hepatocyte-like cells spheroids (i.e. three-dimensional cell culture), both in combination with non-parenchymal cells, into final liver tissue functionality. These results will contribute to improve current liver bioprinting technology towards future regenerative medicine applications and liver physiology and disease modeling.

     

    Learning Objectives:

    • Discuss iPS cell culture and quality control
    • Describe differentiation and functional analysis of relevant liver cells phenotype (ie. hepatocytes, endothelial and MSC)
    • Discuss 3D bioprinting human iPS-derived liver grafts in combination of 3D cultured hepatocyte-like cells

     

     

     

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