Using a Multiplex Suspension Bead Assay to Establish a Serocorrelate of Protection for Group B Streptococcus Disease

Speakers
  • Senior Technician at St George's, University of London

Abstract:

Purpose:
Group B Streptococcus (GBS) is a Gram positive coccus which is part of the normal microbiota of the GI and GU tracts. However GBS is also a major cause of septicaemia and meningitis in newborns. Colonisation with GBS during pregnancy is a major risk factor for neonatal infection. Intrapartum antibiotic prophylaxis has greatly reduced incidence of early onset disease. However concerns over emerging antibiotic resistance and its impact on neonatal intestinal microbiota and a lack of protection afforded by intrapartum antibiotics outside of the first few days of life have led to GBS becoming a candidate for maternal vaccine development. Maternal IgG antibodies targeting capsular polysaccharides have been observed to provide protection against disease (Fabbrini et al 2016). Establishing a serocorrelate of protection could assist in the pathway to licensure of a GBS vaccine.

Methods:
A Bill and Melinda Gates Foundation funded consortium led by Kirsty Le Doare to establish a standardised multiplex immunoassay (MIA), based up luminex’s Xmap technology, and an opsonophagocytic killing assay (OPkA). This will be followed by a inter laboratory study between multiple partners to establish assay parameters and reproducibility.

Results:
Standardised reagents (CPS-PLL conjugates, standards) are being developed and characterised and preparations are being made for the inter laboratory study.

Conclusion:
Identifying a level of IgG which provides protection from GBS disease has been hampered by a lack of assay standardisation. A consortium has been established to develop standardised reagents and assays, including the multiplex immunoassay. Future studies to assess the relationship between antibody level, colonisation and disease are being planned.


Show Resources
You May Also Like
SEP 05, 2019 4:00 PM CEST
C.E. CREDITS
SEP 05, 2019 4:00 PM CEST
DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
JAN 23, 2020 9:00 AM PST
C.E. CREDITS
JAN 23, 2020 9:00 AM PST
DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
OCT 02, 2019 11:00 AM PDT
OCT 02, 2019 11:00 AM PDT
DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
DEC 05, 2019 5:00 AM PST
C.E. CREDITS
DEC 05, 2019 5:00 AM PST
DATE: December 5, 2019TIME: 5:00am PST, 8:00am EST The diagnosis of an active infection by the hepatitis B virus (HBV) relies on testing for the HBV surface antigen (HBsAg). Ov...
APR 20, 2020 8:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
C.E. CREDITS
APR 20, 2020 8:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
This talk will cover different approaches to emergency hemostasis testing for patients that are actively bleeding due to trauma, surgery, obstetrical emergencies and other causes. The talk i...
OCT 31, 2019 9:00 AM PDT
C.E. CREDITS
OCT 31, 2019 9:00 AM PDT
DATE: October 31, 2019TIME: 9:00am PDT, 12:00pm EDT INTRODUCTION: A major limitation for the development of 3D engineered tissues is the absence of viable and perfusable...
Loading Comments...
Show Resources
Event Countdown
  • 0 Days
  • 0 Hours
  • 0 Minutes
  • 0 Seconds