JUL 20, 2016 8:00 AM PDT

Using multiplexed IHC to effectively navigate the hidden clues of the immunological microenvironment

Speaker
  • Field Applications Scientist, PerkinElmer
    Biography
      Dr. Stack completed his undergraduate education at Stony Brook University in Stony Brook, New York prior to his Ph.D. studies at Boston College in Chestnut Hill, Massachusetts. He carried out postdoctoral research at Boston University, where he began to develop his interests in human translational research. Dr. Stack has carried out many oncology studies examining disease mechanisms in multiple cancer types while at the Dana-Farber Cancer Institute, where he was the associate director of the Dana-Farber/Brigham and Women's Center for Molecular Oncologic Pathology, and an instructor in the Pathology Department of Brigham and Women's and the Harvard Medical School. Dr. Stack was also a member of the Dana-Farber/Harvard Cancer Center in both the gastrointestinal malignancies and prostate cancer research programs. Dr. Stack is currently a pathology scientist at PerkinElmer, where he is interested in developing cancer immunology-focused, tissue-based assays, which can provide deeper insights into cancer-host interactions and assist in clinical management of cancer.

    Abstract
    DATE: July 20th
    TIME: 8AM PT, 11AM ET, 5PM CEST


    Therapies like ipilimumab and nivolumab  have shown the potential for approaches that direct the patient’s own immune system against tumors.  Further advances will require a detailed understanding of the tumor microenvironment and characterization of the location and status of immune cells and their interaction with tumor cells.  This will require methods that provide phenotyping of immune and cancer cells combined with the cytoarchitectonics of the tumor. To achieve this, a practical method for simultaneous immunohistochemistry of up to 8 biomarkers in a single tissue section using standard unlabeled primary antibodies will be described. In addition, approaches for multiplexed imaging, single cell quantitative analysis, automated phenotyping and bioinformatics that enable new insights into cancer biology and immunology will be presented. These will then be leveraged in analyses of multiple cancer types, where it is established that the host-tumor interaction is complex and difficult to characterize with standard immunohistochemistry or flow cytometry. But by leveraging multiplexed IHC in situ, we will demonstrate the unique spatial relationships of immune phenotypes in and around both epithelial and non-epithelial tumor types, and show how this data has the potential to form the basis of assays that can guide therapy and monitor response. Further data regarding functional assessment of regulatory immunologic co-factor expression will be discussed.
     

    Show Resources
    You May Also Like
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    MAY 08, 2020 10:00 AM PDT
    C.E. CREDITS
    MAY 08, 2020 10:00 AM PDT
    DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
    FEB 26, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    MAR 31, 2020 7:00 AM PDT
    C.E. CREDITS
    MAR 31, 2020 7:00 AM PDT
    DATE: March 31, 2020 TIME: 7:00am PT, 10:00am PT Please join us for this webinar, during which we will present and discuss the promotion of NK cell-driven tumor immunity. In addition, we wil...
    JUN 23, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 23, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: June 23, 2020 TIME: 10:00am PT Human mesenchymal stromal or stem cells (MSCs)-based immunomodulation treatment has been proposed as a suitable therapeutic approach for many diseases, s...
    Loading Comments...
    Show Resources
    Attendees
    • See more