OCT 11, 2018 10:30 AM PDT

Validating and Publishing with Antibodies- How to Avoid Reviewer's Challenges

Sponsored by: MilliporeSigma
Speaker
  • Senior Manager, Scientific Content and Training, MilliporeSigma
    Biography
      Chandra Mohan received his Ph.D. in 1976 from Bangalore University and did his post-doctoral work from 1977 to 1983 at the University of Southern California, School of Medicine. From 1983 to 1993 he was an Assistant Professor of Pharmacology and Nutrition at the University of Southern California, Medical School in Los Angeles. During this time he also served as an Associate Editor of the journal "Biochemical Medicine and Metabolic Biology." In 1993 he joined Calbiochem (EMD), working as the Director of Technical Services & Training and Senior Technical Writer. In 2013, he was appointed Director, Technical Marketing at EMD Millipore and is currently Senior Manager, Scientific Content and Training at MilliporeSigma. Chandra's research interests include diabetes, biochemical basis of stress, inflammation, and the biology of the aging grain. He is a member of the American Society of Biochemistry and Molecular Biology (ASBMB) and the American Association for the Advancement of Science. Dr. Mohan has more than 45 publications in peer reviewed journals and has delivered over 500 seminars to academic and industrial audiences. You can find his bio featured in Who's Who in America, Who's Who in the West, and Who's Who in Health Care and Medicine.

    Abstract

    Antibodies are extremely valuable and ubiquitous tools in life science research, but in spite of their widespread use in immunoassays over the past several decades, there is still a lack of understanding on the design, validation, and appropriate use of antibodies and controls to publish convincing science. It is generally accepted that a good antibody must be specific, selective, and should provide reproducible results in the context for which it is used. The large diversity of antibody users, their background and experience, their large range of applications, coupled with hundreds of commercial sources for antibodies and immunotechnologies, makes understanding antibody theory and use very relevant today. This presentation will discuss several key aspects of antibody design, validation, and best practices for publishing on various applications from basic immunopurifications and Western blots, to conformation specific binding and high speed imaging flow cytometry. Typical reviewer comments will be addressed and discussed in the context of antibody binding theory and practice. Further, best practices in selecting antibodies, the need for multiple validations, documentation, and commercial source partnerships will be discussed. 

    Learning Objectives:

    1. What are the key elements of antibody design and validation 
    2. How to select the right antibody 
    3. What information is helpful for reviewers 


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