OCT 11, 2018 10:30 AM PDT

Validating and Publishing with Antibodies- How to Avoid Reviewer's Challenges

Sponsored by: MilliporeSigma
Speaker

Abstract

Antibodies are extremely valuable and ubiquitous tools in life science research, but in spite of their widespread use in immunoassays over the past several decades, there is still a lack of understanding on the design, validation, and appropriate use of antibodies and controls to publish convincing science. It is generally accepted that a good antibody must be specific, selective, and should provide reproducible results in the context for which it is used. The large diversity of antibody users, their background and experience, their large range of applications, coupled with hundreds of commercial sources for antibodies and immunotechnologies, makes understanding antibody theory and use very relevant today. This presentation will discuss several key aspects of antibody design, validation, and best practices for publishing on various applications from basic immunopurifications and Western blots, to conformation specific binding and high speed imaging flow cytometry. Typical reviewer comments will be addressed and discussed in the context of antibody binding theory and practice. Further, best practices in selecting antibodies, the need for multiple validations, documentation, and commercial source partnerships will be discussed. 

Learning Objectives:

1. What are the key elements of antibody design and validation 
2. How to select the right antibody 
3. What information is helpful for reviewers 


Show Resources
You May Also Like
NOV 16, 2022 7:00 PM PST
C.E. CREDITS
NOV 16, 2022 7:00 PM PST
Date: November 16, 2022 Time: 2:00pm (AEST) Date: November 17, 2022 7:00pm (PST), 10:00pm (EST), 4:00am (CET) The growth in FDA-approved cell and gene therapy products for the treatment of d...
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
SEP 22, 2022 9:00 AM PDT
SEP 22, 2022 9:00 AM PDT
Date: September 22, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Optimizing platforms for surgical specimen collection and deep human phenotyping was used to enhance protein biomarke...
SEP 29, 2022 11:00 AM PDT
SEP 29, 2022 11:00 AM PDT
Date: September 29, 2022 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEST) Pharmacogenomic (PGx) testing is a dynamic and fascinating application for genetic testing. We'll review the bas...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
OCT 11, 2018 10:30 AM PDT

Validating and Publishing with Antibodies- How to Avoid Reviewer's Challenges

Sponsored by: MilliporeSigma


Show Resources
Loading Comments...
Show Resources