OCT 11, 2018 10:30 AM PDT

Validating and Publishing with Antibodies- How to Avoid Reviewer's Challenges

Sponsored by: MilliporeSigma
Speaker

Abstract

Antibodies are extremely valuable and ubiquitous tools in life science research, but in spite of their widespread use in immunoassays over the past several decades, there is still a lack of understanding on the design, validation, and appropriate use of antibodies and controls to publish convincing science. It is generally accepted that a good antibody must be specific, selective, and should provide reproducible results in the context for which it is used. The large diversity of antibody users, their background and experience, their large range of applications, coupled with hundreds of commercial sources for antibodies and immunotechnologies, makes understanding antibody theory and use very relevant today. This presentation will discuss several key aspects of antibody design, validation, and best practices for publishing on various applications from basic immunopurifications and Western blots, to conformation specific binding and high speed imaging flow cytometry. Typical reviewer comments will be addressed and discussed in the context of antibody binding theory and practice. Further, best practices in selecting antibodies, the need for multiple validations, documentation, and commercial source partnerships will be discussed. 

Learning Objectives:

1. What are the key elements of antibody design and validation 
2. How to select the right antibody 
3. What information is helpful for reviewers 


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
MAR 16, 2021 10:00 AM PDT
C.E. CREDITS
MAR 16, 2021 10:00 AM PDT
Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
APR 01, 2021 8:00 AM PDT
C.E. CREDITS
APR 01, 2021 8:00 AM PDT
Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
MAR 18, 2021 8:00 AM PDT
C.E. CREDITS
MAR 18, 2021 8:00 AM PDT
DATE: March 18, 2021 TIME: 8:00am PDT Sequencing of bulk cells, single cells, and nuclei is opening doors in the understanding of complex biological processes....
APR 21, 2021 5:00 PM CEST
APR 21, 2021 5:00 PM CEST
Date: April 21, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Spatial Answers Trilogy - Spatial Answers in Immunology Immunology Researchers share their Spatial Discoveries in SARS-C...
OCT 11, 2018 10:30 AM PDT

Validating and Publishing with Antibodies- How to Avoid Reviewer's Challenges

Sponsored by: MilliporeSigma

Specialty

Antibodies

Molecular Biology

Immunology

Bioinformatics

Gene Expression

Cancer Diagnostics

Molecular Diagnostics

Cancer Research

Biomarkers

Clinical Research

Gene Sequencing

Digital Pathology

Dna

Personalized Medicine

Biotechnology

Geography

North America80%

Europe20%

Registration Source

Website Visitors100%

Job Title

Student25%

Pathologist25%

Clinical Laboratory Scientist25%

Medical Laboratory Technician25%

Organization

Hospital40%

Academic Institution20%

Pharmaceutical Company20%

Other20%


Show Resources
Loading Comments...
Show Resources
Attendees
  • See more