AUG 22, 2017 9:00 AM PDT

WEBINAR: A virtual workshop for precise HDR-mediated genome engineering with CRISPR-Cas9

Sponsored by: Dharmacon
Speaker

Abstract

DATE: August 22, 2017
TIME: 9:00am PT, 12:00pm ET

The use of CRISPR-Cas9 to create targeted double-strand breaks in genomic DNA has greatly simplified strategies for precise gene editing using the homology-directed repair (HDR) mechanism.  This approach enables knock-in of fluorescent reporters, SNP repair, exact deletions, and many other genomic alterations. However, designing target-specific guide RNAs and DNA donor templates for achieving robust cleavage and repair has many challenges. In addition, the efficiency of HDR-mediated repair is often low. In this workshop, we will demonstrate the use of free, intuitive, online tools to design and order the necessary reagents to overcome these obstacles with HDR-mediated gene editing applications.


Learning Objectives:

  • Considerations for designing ideal guide RNAs and DNA oligo or plasmid repair templates for HDR-mediated CRISPR-Cas9 applications
  • Use of online tools for guide RNA and DNA repair templates for HDR-mediated gene editing applications
  • Optimization of experimental conditions to achieve robust gene editing
  • Demonstration of successful HDR-mediated CRISPR Cas9 experiments
     

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AUG 22, 2017 9:00 AM PDT

WEBINAR: A virtual workshop for precise HDR-mediated genome engineering with CRISPR-Cas9

Sponsored by: Dharmacon

Specialty

Molecular Biology

Cell Biology

Biotechnology

Cancer Research

Genetics

Gene Expression

Cell Culture

Biochemistry

Molecular Diagnostics

Crispr-Cas9

Immunology

Gene Editing

Dna

Genomics

Cancer Diagnostics

Geography

North America52%

Europe24%

Asia12%

South America5%

Oceania2%

Africa2%

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Website Visitors100%

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Research Scientist20%

Student18%

Scientist13%

Post Doc13%

Educator/Faculty11%

Executive7%

Facility/Department Manager5%

Medical Doctor/Specialist4%

Lab Management3%

Medical Laboratory Technician3%

Biologist2%

Clinical Laboratory Scientist2%

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Research Institute8%

Biotech Company6%

Clinical Laboratory2%

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Hospital2%

Pharmaceutical Company1%

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Non-Profit Organization1%

Life Science Company1%

Consultant1%

Contract Research Organization (Cro)1%

Other4%


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