JUL 26, 2016 07:30 AM PDT

Automated assays for protein engineering: in vitro and in vivo

Speakers
  • Postdoctoral Researcher, Biocatalysis, Université de Montréal
    Biography
      Dr. Daniela Quaglia, Ph.D., is a Postdoctoral Researcher in Biocatalysis at Université de Montréal in the laboratory of Prof. Joelle Pelletier. Her current project (co-funded by NSERC and the industrial partner DSM) focuses on the use of enzyme engineering to generate improved biocatalysts to be used in the food industry.
      Dr. Quaglia earned her Bachelor's degree in Chemistry and her Master's in Chemistry of Biological Molecules at the University of Florence in Italy. She then pursued her doctorate in Biocatalysis at University College Dublin, in Ireland where her work focused on the study of the enzyme Horse Liver Alcohol Dehydrogenase and its application in the synthesis of anti-inflammatory drugs. She then moved to the UK, where she worked in industry at Synthace, a Synthetic Biology company in London, then in academia, as a Research Associate in Biocatalysis in the laboratory of Prof. Nicholas Turner at the University of Manchester. Dr. Quaglia has also been involved in teaching at undergraduate level both at the Dundalk Institute of Technology (Ireland) and at the Manchester Metropolitan University. Furthermore, she has a passion for scientific communication: she was recently awarded a prestigious NatureJobs writing competition and a UK Biochemical Society outreach award. Her writing is regularly published in widely-read blogs such as the Huffington Post and you can follow her on Twitter (@Dny_Q).

    Abstract:

    Enzyme engineering is a powerful technology now widely used in laboratories around the world. The goal is to obtain improved proteins that will serve as better biocatalysts, biosensors or as a tool to understand protein evolution, which is at the core of many societal problem, such as antibiotic resistance. Enzyme engineering entails the initial generation of libraries of mutants that will then be screened for improved properties (for example: better selectivity or specificity towards a substrate of interest, improved stability, etc). Obtaining good quality libraries is becoming routine thanks to the availability of molecular biology tools and computational simulations. The bottleneck of the experiment remains at the screening level. The bigger the generated library, the more stringent the requirement for the high-throughput screen. 
    In our work we tackle both aspects: library generation and screening throughput by using the enzyme Candida antarctica lipase A (Cal-A) as a model. Cal-A has the potential to be applied in the food industry for the hydrolysis of saturated short-chain fatty acids (C4 and C6) vs. saturated C10-C16 fatty acids - a very useful tool for the dairy industry. Upon generation of several libraries of mutants using a novel Golden Gate-based strategy, we developed two screening methods (in vivo and in vitro) that give us the required high-throughput capacity. The two screening methods, one carried out on agar plates and the other in liquid, are completely automated and they are both optimized for our Biomek NXP system.


    Show Resources
    You May Also Like
    FEB 26, 2019 09:00 AM PST
    C.E. CREDITS
    FEB 26, 2019 09:00 AM PST
    DATE:  February 26, 2019TIME:   9:00am PST, 12:00pm EST In an era of increasingly high-throughput, large-scale biology, with companies, government and non-prof...
    JUN 05, 2019 05:00 PM CEST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 05, 2019 05:00 PM CEST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    MAY 16, 2019 04:00 PM CEST
    C.E. CREDITS
    MAY 16, 2019 04:00 PM CEST
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    FEB 20, 2019 09:00 AM PST
    C.E. CREDITS
    FEB 20, 2019 09:00 AM PST
    DATE:  February 20, 2019TIME:  9:00am PST  ...
    FEB 27, 2019 08:00 AM PST
    C.E. CREDITS
    FEB 27, 2019 08:00 AM PST
    DATE:  February 27, 2019TIME:  8:00am PST Pluripotent stem cells (PSCs) can form any tissue or cell in the body, and are the ideal starting material to manufacture...
    MAR 05, 2019 09:00 AM PST
    C.E. CREDITS
    MAR 05, 2019 09:00 AM PST
    DATE:  March 5, 2019TIME:  09:00am PST, 12:00pm EST MicroRNA(miRNA) are short non-coding single stranded RNA molecules that regulate gene expression at the post tran...
    Loading Comments...
    Show Resources