MAR 10, 2016 08:00 AM PST

Fully Automated, One Hour Sample Preparation for Ultrafast N-Glycosylation Analysis of Antibody Therapeutics

SPONSORED BY: Sciex, Sciex
  • Applications Manager, SCIEX Separations
      Recipient of the 2016 BioProcess International Best Technology Application - Analytical Award for his work on SCIEX Fast Glycan Technology, András Guttman currently leads the application efforts at Sciex Separations (Brea, CA) and holds the Translational Glycomics Professorship at the Horváth Csaba Laboratory of Bioseparation Sciences (University of Debrecen, Hungary). His work focuses on capillary electrophoresis and CE-MS based proteomics and glycomics analysis of biopharmaceutical, biomedical and cell biology interests. Dr Guttman previously held academic appointments at Northeastern University (Boston, MA) and a Marie Curie Chair at University of Innsbruck (Austria) as well as industrial positions at Novartis (La Jolla, CA), Genetic BioSystems (San Diego, CA), and Beckman Instruments (Fullerton, CA), developing high resolution capillary electrophoresis and microfluidics based separation methods. Professor Guttman has more than 269 scientific publications, wrote 35 book chapters, edited 4 textbooks and holds 19 patents. Until recently he served on the CASSS Board and as president of the Hungarian Chapter of the American Chemical Society. He is on the editorial boards of numerous international scientific journals. Dr Guttman graduated from University of Veszprem, Hungary in chemical engineering, where he also received his doctoral degree. He was recognized by the Analytical Chemistry Award of the Hungarian Chemical Society in 2000, elected as a member of the Hungarian Academy of Sciences in 2004, named as Fulbright Scholar in 2012, received the CASSS CE Pharm Award in 2013, the Arany Janos medal of the Hungarian Academy of Sciences, the Pro Scientia award of the University and the Dennis Gabor Award of the Novofer Foundation in 2014. Dr. Guttman is also the recipient of the 2017 Dal Nogare Award of the Delaware Valley Chromatography Forum, which he received at Pittcon earlier this year.

    DATE: March 10, 2016
    TIME:  8:00am Pacific time, 11:00am Eastern time

    SCIEX has recently introduced an easy-to-use magnetic bead based sample preparation workflow, enabling the preparation of up to 96 samples in one hour. This novel protocol aims to alleviate the hard-to-automate centrifugation and vacuum-centrifugation steps used in other N-glycosylation analysis methods. In this presentation, we demonstrate the next level of this work by fully automating all steps of the optimized magnetic bead based method to eliminate manual sample handling. The one-hour sample preparation protocol for CE-LIF based N-glycosylation analysis of antibody therapeutics includes endoglycosidase digestion, fluorophore labeling and sample clean-up. Automated liquid handling offers fast and precise sample preparation with minimized contamination risks and increased reproducibility to streamline laboratory operations. This rapid sample prep allows you to scale up from single vial analyses to 96 well plates for high throughput screening by using a Laboratory Automation Workstation (Beckman Coulter Biomek FXP®) to accomplish complete sample processing in 60 minutes.

    Learning Objectives:
    •      Introduce the easy-to-use magnetic bead based sample preparation
           workflow for rapid N-glycan analysis of biopharmaceuticals
    •      Describe full automation option of the optimized protocol
    •      Demonstrate ultrafast glycan analysis by capillary electrophoresis in high
           throughput, high resolution and MS friendly formats, respectively

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