JUN 29, 2016 7:30 AM PDT

Charge Heterogeneity Analysis of Biologics by Capillary Isoelectric Focusing (cIEF) and Capillary Zone Electrophoresis (CZE)

Sponsored by: Sciex, Sciex
Speaker
  • Manager, CE Field Applications Support, SCIEX Separations
    Biography

           Brandon Bates is currently the Field Applications Support Manager with Sciex Separations. He began his CE customer support career with Beckman Coulter in 2010, transitioning to Sciex in 2014. During this six year period, Brandon had overseen the installation and successful implementation of every Sciex CE instrument in the Central US through onsite support and training. He also oversees all CE applications specialists in the US.
           Prior to his time with Sciex Separations, he gained experience while employed at the University of Iowa department of Internal Medicine, followed by a position with the Department of Microbial Systems Biology at Argonne National Laboratory.


    Abstract
         Implementation of Capillary Electrophoresis (CE) technology in the biopharmaceutical industry is helping to characterize purity, heterogeneity, and identity of therapeutic proteins. Furthermore, the charge heterogeneity profile of protein samples can be revealed through CE, with excellent resolution. Determine protein isoform charge heterogeneity with high speed, high resolution capillary isoelectric focusing (cIEF) and zone electrophoresis (CZE).
         cIEF is a powerful technique that allows for ultra-high resolution and quantitative analysis of proteins separated based on isoelectric point (pI). Alternatively, you can obtain high resolution charge heterogeneity information more rapidly with reduced sample preparation, using Capillary Zone Electrophoresis.
         Using fully optimized methods and synthetic pI markers in cIEF attains the highest levels of precision in pI determination and direct isoform quantitation. The PA 800 Plus Pharmaceutical Analysis System enhances these applications by providing excellent intermediate precision with pI reproducibility < 1% (cIEF), peak migration time reproducibility < 3% (CZE) and peak area reproducibility < 3% (cIEF/CZE). Join us to discover the advantages of cIEF & CZE methods for charge heterogeneity applications.

    Learning Objectives:
    • Basic fundamentals of charge heterogeneity CE techniques 
    • Introduction to our two distinct charge heterogeneity applications: cIEF and CZE
    • Advantages of each charge heterogeneity technique

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