APR 06, 2017 7:00 AM PDT

WEBINAR: Colony picking in a fully automated DNA assembly workflow

Sponsored by: Molecular Devices
Speakers

Abstract

DATE:  April 6, 2017
TIME:  3:00PM GMT, 7:00AM PT, 10:00AM ET

The Edinburgh Genome Foundry (EGF) is a newly established facility of the University of Edinburgh and assembles built-to-order DNA constructs using a fully integrated robotic platform. The assembly of synthetic DNA has become a standard process in many scientific laboratories. The assembly protocols involve many repetitive steps and often scientists require many different versions of DNA. Therefore, this process is well suited for automation, which enables the high-throughput needed and also resulting in a lower error rate and cost saving for reagents and consumables.

Colony Picking is a key part of the synthetic biology workflow, where bacteria are used to propagate cloned DNA, or to screen DNA constructs against selective conditions. Yet, at high throughput (typically thousands of colonies per day) the task becomes too labor-intensive and error-prone to be performed by hand.

In this webinar we will describe our robotic platform and how it performs automated DNA assembly using the Golden Gate DNA assembly method. Furthermore, we present how the Molecular Devices QPix integrates into the automated production line and is used for routine high-throughput colony picking/plating. We will describe the customization of the instrument itself, as well as our collaborative efforts with Molecular Devices and our systems integrator to integrate the QPix into our extensive lab automation setup.

Learning Objectives:

  • Learn about the automation of DNA assembly, as exemplified using the Golden Gate assembly method

  • Learn about the customization and integration of high-throughput colony picking/plating


Show Resources
You May Also Like
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
NOV 16, 2022 7:00 PM PST
C.E. CREDITS
NOV 16, 2022 7:00 PM PST
Date: November 16, 2022 Time: 2:00pm (AEST) Date: November 17, 2022 7:00pm (PST), 10:00pm (EST), 4:00am (CET) The growth in FDA-approved cell and gene therapy products for the treatment of d...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
SEP 13, 2022 8:00 AM PDT
SEP 13, 2022 8:00 AM PDT
Date: September 13, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Preparedness against pandemic diseases demands rapid-response vaccine technology and ready-to-use analytical methods...
SEP 22, 2022 9:00 AM PDT
SEP 22, 2022 9:00 AM PDT
Date: September 22, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Optimizing platforms for surgical specimen collection and deep human phenotyping was used to enhance protein biomarke...
APR 06, 2017 7:00 AM PDT

WEBINAR: Colony picking in a fully automated DNA assembly workflow

Sponsored by: Molecular Devices


Show Resources
Loading Comments...
Show Resources