WEBINAR: ProQuantum high-sensitivity immunoassays offer minimum sample consumption with maximum performance

Speaker
  • Senior R&D Manager and Immunoassay Strategy Lead, Biosciences Division, Thermo Fisher Scientific
    Biography
      David's Ph.D. and postdoctoral work focused on the study of G protein-coupled receptor signaling and thrombosis. Following his academic training, he led multiplexed immunoassay automation efforts at a Luminex® partnering company. In 2009, David joined Thermo Fisher Scientific working on the development of novel immunoassay platforms such as the ProtoPlex™ Immune Response Assay. Now as Sr. Manager and immunoassay strategy lead, David pursues next-gen immunoassay platform development which includes working with translational investigators to identify serum-based biomarkers in cancer, autoimmunity, and inflammation.

    Abstract

    Early detection of protein biomarkers is critical to the study of human diseases, yet current tools available for low-level protein quantitation do not address all the needs for protein and molecular biologists alike. A common concern is sample volume limitation due to difficulties in obtaining sufficient biological samples from various models in research studies. Additionally, cost and time-to-answer are always a factor when considering which assay platform to use. In many cases, end users must make trade-offs on which factors impact their work the least.

    Invitrogen™ ProQuantum™ high-sensitivity immunoassays combine multiple benefits into a single platform so that users don’t need to make trade-offs. The platform works by combining the analyte specificity of high-affinity antibody–antigen binding with the signal detection and amplification of real-time PCR. The result is an assay that can measure low-expressing proteins (femtogram levels) while only consuming a fraction of sample volume (i.e.,1–2 microliters per sample) compared to other immunoassay methods. Best of all, it uses a streamlined workflow (2 hours) that is highly scalable and affordable on an open, widely used qPCR platform.

     

    Learning Objectives:

    • To understand the multiple benefits of ProQuantum high-sensitivity immunoassays and how the platform works
    • To understand how the kits are designed for a simplified workflow amenable to high-throughput applications

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