WEBINAR: Signaling Pathways at Your Fingertips - Multiplex IP to Targeted Mass Spectrometry Assay Kits for AKT/mTOR Pathway

Speaker
  • Senior R&D Scientist, Protein and Cell Analysis, Thermo Fisher Scientific
    Biography
      Bhavin Patel works as a senior R&D scientist in mass spectrometry reagents group at Thermo Fisher Scientific. Bhavin has an MD degree from the Maharaja Sayajirao University of Baroda in India, and an MS in biotechnology from the Georgetown University. Bhavin had led a protein biomarker assay group at NextGen Sciences and a proteomics/mass spectrometry group at Fox Chase Cancer Center before joining Thermo Fisher Scientific in 2013. Since joining Thermo Fisher Scientific, Bhavin has led the development of targeted mass spectrometry (MS) assay kits, new MS standards, protein sample preparation reagents, an antibody validation method for IP-MS, and reagents for quantitative proteomic analysis.

    Abstract

    Proteomics offers a deeper understanding of the protein events influencing cellular and biological function. However, identifying key regulated proteins and their implicated signaling pathways represents a major analytical challenge to researchers. This is due to the broad dynamic range of protein abundance in biological samples and the inherent limitations of the instrumentation and methodologies utilized to probe and detect minute target proteins in complex cellular environments. In this webinar, we discuss targeted mass spectrometry (MS)-based approaches and the various strategies designed to reduce sample complexity through improved sample preparation and enrichment. Focusing on proteins involved in the AKT/mTOR signaling pathway, we provide guidance toward targeted MS assay development and validation, including robustness, precision, and accuracy of these assays. Finally, we’ll examine results of multiplexed IP to targeted MS assays compared to existing immunoassay techniques.

    • Development and validation of immunoenrichment to mass spectrometry (IP-MS) assays
    • Accurate and precise quantitation of multiple AKT/mTOR pathway proteins by multiplex IP to targeted MS assays and comparison to exiting immunoassay techniques

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