FEB 28, 2020 9:00 AM PST

Widely Applicable Charge Variant Analysis of Therapeutic Proteins

Sponsored by: Sciex
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Applications Scientist, Sciex
    Biography
      Peter Holper obtained his degree in Biochemistry and Cell Biology from the University of California - San Diego. Peter has over 12 years of experience in biopharma, including as an analytical chemist at Eli Lilly and Company where he was responsible for developing the analytical control strategy for bioproducts. Peter currently works at SCIEX in Redwood City, CA (US) where he is responsible for developing and optimizing CE applications and providing customer demo sample analysis support.

    Abstract
    DATE: February 28, 2020
    TIME: 9:00am PST
     
     
    Finding ways to bring therapeutic proteins to market faster is an ongoing challenge for global pharmaceutical development. Of all the analytical methods to be developed, charge variant analysis methods, used to monitor post-translational modifications (PTMs), generally require the longest development time.
     
    Join Peter Holper from SCIEX to discover a new approach to finding a robust, stability indicating charge variant method. Using a widely universal buffer, this technique requires little to no method development, has a simple sample preparation, analyzes a molecule in its native state, and has a higher analytical throughput than IEX or icIEF. 
     
    Learning Objectives:
    • Reproducible: CZE separations are shown to be reproducible through both replicate injections of commercial mAbs and an inter-company collaboration.
    • Platform capable: The simplicity of separation and optimal pH of the buffer allows for application to molecules across a broad pI range above 7.0.2
    • Limited method development: Additional resolution can easily be obtained through the use of a longer bare-fused silica capillary, lower capillary temperature or separation voltage. Further development has focused on buffer pH and levels of additives.
    • High-Throughput: High-resolution separation can be achieved in approximately 5 minutes or less. With only two minutes of buffer replenishment in between injections, total sample analysis time can be completed in under 10 minutes.
     
    Webinars will be available for unlimited on-demand viewing after live event.
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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